Study on the Extraction and Purification of Microcystins (MC-LR)

Wen Yi Zhang; Ning Han; Li Rong Yao; Xiao Lan Qiu; Xiao Liang Chen

doi:10.4028/www.scientific.net/AMR.340.318

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Improvement of Shake Flask Fermentation Conditions for the Pseudomonas Strain N1
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GH002: A Novel and Potent Agents for Elevating HDL-Cholesterol
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Fermentation Conditions Optimization of Anti-Tumor Active Metabolites from Marine Penicillium sp. HGQ6
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 340Study on the Extraction and Purification of...

Study on the Extraction and Purification of Microcystins (MC-LR)

Abstract:

The MC-LR from the the blue-green algae of Taihu Lake was extracted, at the same time, a set of microcystins extraction method with methanol as extraction solvent and purification method with C18-SPE as purification workstations were established. The extraction solvent concentration, extraction time, extraction solvent amount, leacheate concentration and eluent concentration were used to research the extraction efficiency of MC-LR. Finally, 80% methanol was used to wash microcytins to make MC-LR high purity and the purity was over 85%. This research presented a method of low cost and high efficiency. It provided the foundation for the further research of microcytins.

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Periodical:

Advanced Materials Research (Volume 340)

Pages:

318-323

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.340.318

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Online since:

September 2011

Authors:

Wen Yi Zhang, Ning Han, Li Rong Yao, Xiao Lan Qiu, Xiao Liang Chen

Keywords:

Extraction, High Performance Liquid Chromatography (HPLC), Microcystins, Purification

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References

[1] ZHANG Wei-hao, XU Xiao-qing, QIU Chang-qiang. Advance in Study on Microcystins in Aquatic Environment[J]. Research of Environmental Sciences, 2001, 14(2): pp.57-61.

Google Scholar

[2] Duy T.N., Lam P.K.S., Shaw G. R. Toxicology and risk assessment of fresh water cyanobacterial(blue-green algae) toxins in water. Rev. Environ. Contam. Toxicol., 2000, 163: pp.113-118.

DOI: 10.1007/978-1-4757-6429-1_3

Google Scholar

[3] Shen P P, Shi Q, Hua Z C, Analysis of microcystins in cyanohacteria bloom and surface water samples from Meiliang Bay, Taihu Lake, China, J. Environment International, vol. 29 , 2003, pp.641-647.

DOI: 10.1016/s0160-4120(03)00047-3

Google Scholar

[4] YU Shun-zhang, ZHAO Ning, ZI Xiao-lin, et al, The relation ship between cyanotoxin (Microcystin, MC) in pond ditch water and primary liver cancer in China, J. Chinese Journal of Oncology, vol. 23, 2001, pp.96-99.

Google Scholar

[5] ZHOU Lun, YU Da, YU Hai, et al, Drinking water types, microcystins and colorectal cancer, J. Chinese Journal of Preventive Medicine, vol. 34, 2000, pp.224-226.

Google Scholar

[6] Falconer I. R, An overview of problems caused by toxic blue-green algac(cyanobacteria) in drinking and recreational water, J. Environmental Toxicol., vol. 14, 1999, pp.5-12.

DOI: 10.1002/(sici)1522-7278(199902)14:1<5::aid-tox3>3.0.co;2-0

Google Scholar