Study on the Extraction and Purification of Microcystins (MC-LR)
The MC-LR from the the blue-green algae of Taihu Lake was extracted, at the same time, a set of microcystins extraction method with methanol as extraction solvent and purification method with C18-SPE as purification workstations were established. The extraction solvent concentration, extraction time, extraction solvent amount, leacheate concentration and eluent concentration were used to research the extraction efficiency of MC-LR. Finally, 80% methanol was used to wash microcytins to make MC-LR high purity and the purity was over 85%. This research presented a method of low cost and high efficiency. It provided the foundation for the further research of microcytins.
W. Y. Zhang et al., "Study on the Extraction and Purification of Microcystins (MC-LR)", Advanced Materials Research, Vol. 340, pp. 318-323, 2012