Fermentation Conditions Optimization of Anti-Tumor Active Metabolites from Marine Penicillium sp. HGQ6

Guo Lei; Wen Cheng Zhu; Chao Liu

doi:10.4028/www.scientific.net/AMR.340.344

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 340Fermentation Conditions Optimization of Anti-Tumor...

Fermentation Conditions Optimization of Anti-Tumor Active Metabolites from Marine Penicillium sp. HGQ6

Abstract:

The objectives of this study were to optimize fermentation conditons of anti-tumor active metabolites from marine penicillium sp. HGQ6. Based on the inhibition activity against stomach cancer cell BGC823, firstly, the initial medium was screened, then seven factors including peptone, sucrose, K₂HPO₄, FeSO₄, pH, temperature and agitation were investigated by Plackett-Burman design, and the important variables including pH, temperature and K₂HPO₄ were optimized by using Response surface methodology (RSM). Experimental results showed that the optimum fermentation conditions were peptone 1%, sucrose 3%, K₂HPO₄ 0.06%, FeSO₄ 0.001%, pH6.41, temperature 35°C and agitation 180 r·min^-1. Under these optimal conditions, the actual inhibition ratio achieved 90.4%, which agreed with the predicted values. This indicated the good feasibility of Plackett-Burman design and RSM in the optimization of fermentation conditions for production of anti-tumor active substances from stain HGQ6.

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Periodical:

Advanced Materials Research (Volume 340)

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344-350

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.340.344

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September 2011

Authors:

Guo Lei, Wen Cheng Zhu, Chao Liu

Keywords:

Anti-Tumor Activity, Fermentation Condition, Marine Fungus, Plackett-Burman, Response Surface Method (RSM)

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