Proteomics Study on Cross-Linking Reaction of Formaldehyde and HSA

Abstract:

Possible clinical diagnosis biomarkers of diabetes were proposed using proteomics and bioinformatics methods through the preliminary study on the formaldehyde-human serum albumin crosslinkage. Using LC/MS techniques, the experiment on in vivo cross-linking reaction of formaldehyde with pure protein or plasma protein revealed that the cross-linking reaction of human serum albumin and forrmaldehyde would occur and the concentrations of some normal peptides would change. A group of significant peptides of human serum albumin were selected. As the results of in vivo cross-linking reaction, Non-enzymatic glycosylation modification of HSA were observed in diabetic. The same change of the concentrations of some peptides happened. The above-mentioned results were demonstrated with the hematoplasmas of normal people and diabetics.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 1035-1041
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.1035
• Online since: September 2011
• Authors: Bo Li, Ding Yu Hu, Xiu Lan Xin, Rong Lan, Yu Lin Deng
• Keywords: Diabetes, Glycosylation, HSA, LC/MS

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