Apoptosis of Human Gastric Cancer Cell Line SGC-7901 Induced by Allicin

Abstract:

To observe the effects of allicin on the growth and apoptosis of gastric cancer cell line. The viability of gastric cancer cell line SGC-7901 was determined by MTT, cell morphology was observed under AO staining and electron microscopy, the apoptosis was measured using flow cytometry analysis. The results indicated that the growth of gastric cancer cell line SGC-7901 was significantly inhibited by allicin, and the IC50 was 80µg/mL. Morphological variations of apoptosis were observed at 48 hours treated with allicin by AO staining and apoptosis body was observed under electron microscope. The results indicated that allicin can suppress the growth of gastric cancer cell line SGC-7901 and induce apoptosis of the cells.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 1285-1289
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.1285
• Online since: September 2011
• Authors: Shu Li Shao, Wei Wei Zhang, Feng Ying Li
• Keywords: Allicin, Apoptosis, Gastric Cancer Cell Line SGC-7901

References

[1] Agarwal KC. Therapeutic actions of garlic constituents. Med Res Rev, vol. 16, no. 1, pp.111-124, January (1996).

DOI: 10.1002/(sici)1098-1128(199601)16:1<111::aid-med4>3.0.co;2-5

[2] Hirsch K, Danilenko M, Giat J, Miron T, Rabinkov A, Wilchek M, et al. Effect of purified allicin, the major ingredient of freshly crushed garlic, on cancer cell proliferation. Nutr Cancer, vol. 38, no. 2, pp.245-254, (2000).

DOI: 10.1207/s15327914nc382_14

[3] Patya M, Zahalka MA, Vanichkin A, Rabinkov A, Miron T, Mirelman D, et al. Allicin stimulates lymphocytes and elicits an antitumor effect: a possible role of p21ras. Int Immunol, vol. 16, no. 2, pp.275-281, February (2004).

DOI: 10.1093/intimm/dxh038

[4] Rabinkov A, Miron T, Konstantinovski L, Wilchek M, Mirelman D, Weiner L. The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins. Biochim Biophys Acta, vol. 1379, no. 2, pp.233-244, February (1998).

DOI: 10.1016/s0304-4165(97)00104-9

[5] Miron T, Rabinkov A, Mirelman D, Wilchek M, Weiner L. The mode of action of allicin: its ready permeability through phospholipid membranes may contribute to its biological activity. Biochim Biophys Acta, vol. 1463, no. 1, pp.20-30, January (2000).

DOI: 10.1016/s0005-2736(99)00174-1

[6] Freeman F, Kodera Y.  Garlic chemistry: stability of S-(2-Propenyl)-2-Propene-1- Sulfinothioate (Allicin) in blood, solvent a) and simulated physiological fluids . J Agri Food Chem, vol. 43, pp.2332-2338, (1995).

DOI: 10.1021/jf00057a004

[7] Arditti FD, Rabinkov A, Miron T, Reisner Y, Berrebi A, Wilchek M, et al. Apoptotic killing of B-chronic lymphocytic leukemia tumor cells by allicin generated in situ using a rituximab-alliinase conjugate. Mol Cancer Ther, vol. 4, no. 2, pp.325-331, February (2005).

DOI: 10.1158/1535-7163.325.4.2

[8] Borek C. Antioxidant health effects of aged garlic extract. J Nutr, vol. 131, no. 3s, pp. 1010s-1015s, March (2001).

DOI: 10.1093/jn/131.3.1010s

[9] Lawson LD, Hughes BG. Characterization of the formation of allicin and other thiosulfinates from garlic. Planta Med, vol. 58, no. 4, pp.345-350, August (1992).

DOI: 10.1055/s-2006-961482

[10] .

[10] Oommen S, Anto RJ, Srinivas G, Karunagaran D. Allicin (from garlic) induces caspase-mediated apoptosis in cancer cells. Eur J Pharmacol, vol. 485, no. 1-3, pp.97-103, February (2004).

DOI: 10.1016/j.ejphar.2003.11.059

[11] Vainio H, Weiderpass E. Fruit and vegetables in cancer prevention. Nutr Cancer, vol. 54, no. 1, pp.111-142, (2006).

DOI: 10.1207/s15327914nc5401_13

[12] Balasenthil S, Rao KS, Nagini S. Altered cytokeratin expression during chemoprevention of experimental hamster buccal pouchcarcinogenesis by garlic. J Oral Pathol Med, vol. 31, no. 3, pp.142-146, March (2002).

DOI: 10.1034/j.1600-0714.2002.310303.x

[13] Kwon SK, Moon A. Synthesis of 3-alkylthio-6-allylthiopyridazine derivatives and their antihepatocarcinoma activity. Arch Pharm Res, vol. 28, no. 4, pp.391-394, April (2005).

DOI: 10.1007/bf02977666

[14] Thatte U, Bagadey S, Dahanukar S. Modulation of programmed cell death by medicinal plants. Cell Mol Biol, vol. 46, no. 1, pp.199-214, February (2000).

[15] Jakubíková J, Sedlák J. Garlic-derived organosulfides induce cytotoxicity, apoptosis, cell cycle arrest and oxidative stress in human colon carcinoma cell lines. Neoplasma, vol. 53, no. 3, pp.191-199, (2006).

[16] Terry MB, Gaudet MM, Gammon MD. The epidemiology of gastric cancer. Semin Radiat Oncol, vol. 12, no. 2, pp.111-127, April (2002).

[17] Li C, Oh SJ, Kim S, Hyung WJ, Yan M, Zhu ZG, et al. Risk factors of survival and surgical treatment for advanced gastric cancer with large tumor size. J Gastrointest Surg, vol. 13, no. 5, pp.881-885, May (2009).

DOI: 10.1007/s11605-009-0800-3

[18] Williams MJ, Sutherland WH, McCormick MP, Yeoman DJ, de Jong SA. Aged garlic extract improves endothelial function in men with coronary artery disease. Phytother Res, vol. 19, no. 4, pp.314-319, April (2005).

DOI: 10.1002/ptr.1663

[19] Gonen A, Harats D, Rabinkov A, Miron T, Mirelman D, Wilchek M, et al. The antiatherogenic effect of allicin: possible mode of action. Pathobiology, vol. 72, no. 6, pp.325-334, (2005).

DOI: 10.1159/000091330

[20] Li W, Wang D, Song G, Zuo C, Qiao X, Qin S. The effect of combination therapy of allicin and fenofibrate on high fat diet-induced vascular endothelium dysfunction and liver damage in rats. Lipids in Health and Disease, vol. 9, no. 1, p.131, November (2010).

DOI: 10.1186/1476-511x-9-131