Enzymatic Synthesis of both Enantiomers of Ethyl 4-Cyano-3-Hydroxybutanoate
(R)-Ethyl 4-cyano-3-hydroxybutanoate is the key intermediate for the synthesis of liptor used for lowering high cholesterol and triglycerides. Firstly both enantiomers of ethyl 4-cyano-3-hydroxybu- tanoate were prepared by the enzymatic reduction of ethyl 4-cyano-3-oxobutanoate with two whole cells in high ee and high yield. The (R)-enantiomer was obtained in 89.8% yield and 98.5% ee in the catalysis of 20 mM substrate by the strain Bacillus pumilus Phe-C3; the (S)-enantiomer was achieved in 83.1% yield and 95.4% ee in the catalysis of 10 mM substrate by the strain Klebsiella pneumomiae Phe-E4. Preparative synthesis were carried out on 200-L scale.
J. Z. Jin and J. Zhang, "Enzymatic Synthesis of both Enantiomers of Ethyl 4-Cyano-3-Hydroxybutanoate", Advanced Materials Research, Vols. 343-344, pp. 448-452, 2012