Enzymatic Synthesis of both Enantiomers of Ethyl 4-Cyano-3-Hydroxybutanoate

Abstract:

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(R)-Ethyl 4-cyano-3-hydroxybutanoate is the key intermediate for the synthesis of liptor used for lowering high cholesterol and triglycerides. Firstly both enantiomers of ethyl 4-cyano-3-hydroxybu- tanoate were prepared by the enzymatic reduction of ethyl 4-cyano-3-oxobutanoate with two whole cells in high ee and high yield. The (R)-enantiomer was obtained in 89.8% yield and 98.5% ee in the catalysis of 20 mM substrate by the strain Bacillus pumilus Phe-C3; the (S)-enantiomer was achieved in 83.1% yield and 95.4% ee in the catalysis of 10 mM substrate by the strain Klebsiella pneumomiae Phe-E4. Preparative synthesis were carried out on 200-L scale.

Info:

Periodical:

Advanced Materials Research (Volumes 343-344)

Edited by:

David Wang

Pages:

448-452

DOI:

10.4028/www.scientific.net/AMR.343-344.448

Citation:

J. Z. Jin and J. Zhang, "Enzymatic Synthesis of both Enantiomers of Ethyl 4-Cyano-3-Hydroxybutanoate", Advanced Materials Research, Vols. 343-344, pp. 448-452, 2012

Online since:

September 2011

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$35.00

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