Impacts of Sodium Citrate on Metabolic Flux Distributions of L-Valine Fermentation

Abstract:

The effect of sodium citrate on the metabolic flux distributions in the middle and late periods of L-valine production by Corynebacterium glutamicum XV0505 was obtained. It was shown that when sodium citrate (2.0 g/L) was added into the initial fermentation culture medium, the metabolic flux of Embden-Meyerhof-Parnas (EMP) route decreased from 96.43 to 91.13, and the metabolic flux of Hexose Monophophate (HMP) route increased from 3.56 to 8.87, and the metabolic flux flowing to L-alanine and acetate was decreased by 21.1% and 32.4%, respectively. Meanwhile, the metabolic flux of biosynthesis route of L-valine was increased by 10.74%. Therefore, sodium citrate can change the metabolic flux distribution in the key nodes of biosynthesis route of L-valine, decrease the generation of byproducts, and increase the metabolic flux in the biosynthesis route of L-valine.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 643-648
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.643
• Online since: September 2011
• Authors: Qing Yang Xu, Lei Ma, Xi Xian Xie, Ning Chen, Jian Wang
• Keywords: L-Valine, Metabolic Flux Analysis, Sodium Citrate

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