In this study, we aimed to detect the single nucleotide polymorphism (SNPs) of the chicken PRKAG3 gene and discern the potential association with meat quality traits. A total of 240 meat-type quality chickens were screened by using single-strand conformational polymorphism analysis (SSCP) and DNA sequencing. Two SNPs (g.1744G>C and g.3207A>G) were identified in chicken PRKAG3 with medium polymorphism (0.25C were significantly associated with water content, crude protein, and crude ash (P<0.05/0.01). The SNP genotypes g.3207A>G were significantly associated with water content, and crude protein (P<0.01). The results suggested that PRKAG3 gene is potential major genes or is in close linkage disequilibrium with the QTL affecting meat quality traits in this population of chickens.