Effect of CuO Nanoparticles on the Cell Membrane Permeability of A549 and its Exclusion

Abstract:

This study was conducted to explore the effect of CuO nanoparticles (NPs) on the cell membrane permeability and its exclusion from cells. Human A549 lung cells were exposed to 5mg/L and 15mg/L CuO NPs. Cell membrane permeability was evaluated in 2h and 4h. After 4 hours exposure, the membrane was damaged. Exclusion of copper from cells after 24h exposure with 5mg/L and 15 mg/L CuO NPs are time and dose dependent. And the cell viability was resumed gradually. It is concluded that CuO NPs could induce cytotoxicity, and destroy the membrane integrity. One detoxify mechanism was the exclusion of excessive copper from cells themselves.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 77-80
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.77
• Online since: September 2011
• Authors: Xiao Yan Xie, Li Li Xu, Dong Mei Gao
• Keywords: A549, Cell Membrane Permeability, CuO Nanoparticles, Exclusion

References

[1] Maynard, A.D., 2006. Nanotechnology: A research strategy for addressing risk. WoodrowWilson International Center for Scholars, Washington, DC.

[2] Zhu, J., Li, D., Chen, H., Yang, X., Lu, L., Wang, X., 2004. Highly dispersed CuO nanoparticles prepared by a novel quick-precipitation method. Materials Letters 58, 3324-3327.

DOI: 10.1016/j.matlet.2004.06.031

[3] Blinova, I., Ivask, A., Heinlaan, M., Mortimer, M., Kahru, A., 2010. Ecotoxicity of nanoparticles of CuO and ZnO in natural water. Environmental Pollution 158, 41-47.

DOI: 10.1016/j.envpol.2009.08.017

[4] Yang, S., Liu, J., Wang, J., Yuan, Y., Cao, A., Wang, H., Liu, Y., Zhao, Y., 2010. Cytotoxicity of zinc oxide nanoparticles: Importance of microenvironment. Journal of Nanoscience and Nanotechnology 10, 8638–8645.

DOI: 10.1166/jnn.2010.2491

[5] Slowing, I., Trewyn, B., Lin, V., 2006. Effect of surface functionalization of MCM-41-Type mesoporous silica nanoparticles on the endocytosis by Human Cancer Cells: J. Am. Chem. Soc. 128 (46), 14792–14793.

DOI: 10.1021/ja0645943

[6] Chavanpatil, M., Khdair, A., Panyam, J., 2006. Nanoparticles for cellular drug delivery: mechanisms and factors influencing delivery. J. Nanosci. Nanotechnol. 6 (9-10), 2651–2663.

DOI: 10.1166/jnn.2006.443

[7] Piao, M., Kang, K., Lee, I., Kim, H., Kim, S., Choi, J., Choi, J., and Hyun, J., 2011. Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol. Lett. 201, 92-100.

DOI: 10.1016/j.toxlet.2010.12.010

[8] Li, S., Zhu, R., Zhu, H., Xue, M., Sun, X., Yao, S., Wang, S., 2008. Nanotoxicity of TiO2 nanoparticles to erythrocyte in vitro, - Food. Chem. Toxicol. 46, 3626–3631.

DOI: 10.1016/j.fct.2008.09.012

[9] Jin, C., Zhu, B., Wang, X., Lu, Q., 2008. Cytotoxicity of Titanium Dioxide Nanoparticles in Mouse Fibroblast Cells, Chem. Res. Toxicol. 21, 1871–1877.

DOI: 10.1021/tx800179f