Grx1 Antagonized High Glucose-Induced Apoptosis in Endothelial Cells through Inhibition of Jnk Pathway

Abstract:

To investigate the mechanism underlying the protective effects of glutaredoxin-1 (Grx1) against high glucose-induced apoptosis in umbilical vein endothelial cells. The proliferation of cells was measured by MTT assay. The cells ultra-structure were observed by TEM and the apoptotic rate was detected by the immunofluorescent of Annexin V－FITC/PI with flow cytometer. The level of p-JNK and JNK were evaluated by western bloting. Results showed that Grx1 prevented the inhibitory effect on cell viability induced by high glucose; Grxl could inhibit high glucose-induced apoptosis and restrain apoptosis rate of endothelial cell significantly. The expression level of p-JNK protein significantly increased while that of JNK protein has no insignificantly chang in cells of the high glucose group, After pretreatment with Grx1, the expression level of p-JNK protein decreased. These results demonstrated that Grx1 has protective effects against high glucose-induced apoptosis in HUVECs through inhibition of JNK pathway.

• Periodical: Advanced Materials Research (Volume 345)
• Pages: 257-262
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.345.257
• Online since: September 2011
• Authors: Hai Tao Yu, Li Ling Yue, Chun Jing Zhang
• Keywords: Apoptosis of Endothelial Cell, Glutaredoxin 1, High Glucose, Signal Transduction

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