Grx1 Antagonized High Glucose-Induced Apoptosis in Endothelial Cells through Inhibition of Jnk Pathway

Abstract:

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To investigate the mechanism underlying the protective effects of glutaredoxin-1 (Grx1) against high glucose-induced apoptosis in umbilical vein endothelial cells. The proliferation of cells was measured by MTT assay. The cells ultra-structure were observed by TEM and the apoptotic rate was detected by the immunofluorescent of Annexin V-FITC/PI with flow cytometer. The level of p-JNK and JNK were evaluated by western bloting. Results showed that Grx1 prevented the inhibitory effect on cell viability induced by high glucose; Grxl could inhibit high glucose-induced apoptosis and restrain apoptosis rate of endothelial cell significantly. The expression level of p-JNK protein significantly increased while that of JNK protein has no insignificantly chang in cells of the high glucose group, After pretreatment with Grx1, the expression level of p-JNK protein decreased. These results demonstrated that Grx1 has protective effects against high glucose-induced apoptosis in HUVECs through inhibition of JNK pathway.

Info:

Periodical:

Edited by:

J.L. Zhong

Pages:

257-262

DOI:

10.4028/www.scientific.net/AMR.345.257

Citation:

H. T. Yu et al., "Grx1 Antagonized High Glucose-Induced Apoptosis in Endothelial Cells through Inhibition of Jnk Pathway", Advanced Materials Research, Vol. 345, pp. 257-262, 2012

Online since:

September 2011

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Price:

$35.00

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