The Effect of Overexpression of α (1, 3)- FucosyltransferaseVII on Adhesive Capability of Human Colon Carcinoma HT-29 Cells to HUVECs

Abstract:

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There are sufficient evidences that Lewis antigens are tumor-associated molecules and correlated to high grade and poor prognosis tumors. In this study, we investigated the effect of (α1,3)-fucosyltransferase VII overexpression on the synthesis of sLex and adhesive capability of human colon carcinoma HT-29 cells to HUVECs.The pIRES2-EGFP-FucT VII eukaryotic expression vector were transiently transfected into HT-29 cells. The changes of FucT VII protein and mRNA expression were determined by flow cytomet- ry and Real-Time PCR; the effect of FucT VII overexpression on synthesis of its downstream product-sLex is detected by Flow cytometry; Rose-Bengal method is used to assay the capability of HT-29 cell adhesion to HUVECs. Results: Eukaryotic expression vector pIRES2-EGFP-FucT VII was successfully transfected into HT-29 cells and made FucT VII overexpressed; compared with that of control group, expression level of the sLeX on the surface of FucT VII transfected HT-29 cells was significantly higher; FucT VII overexpression could enhance the adhesive capability of HT-29 cells to HUVECs. Our data suggest that overexpression of FucT VII could strengthen adhesion of sLeX-mediated HT-29 cells to HUVECs through upregulating sLeX synthesis.

Info:

Periodical:

Edited by:

J.L. Zhong

Pages:

250-256

DOI:

10.4028/www.scientific.net/AMR.345.250

Citation:

L. L. Yue et al., "The Effect of Overexpression of α (1, 3)- FucosyltransferaseVII on Adhesive Capability of Human Colon Carcinoma HT-29 Cells to HUVECs", Advanced Materials Research, Vol. 345, pp. 250-256, 2012

Online since:

September 2011

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$35.00

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