Novel Approach to Fabricate an Amperometric Biosensor for Glucose Based on Nafion, Azure І and Gold Nanoparticles

Yong Zhou

doi:10.4028/www.scientific.net/AMR.345.331

Paper Titles

The Preparation of Carbon Nanotubes (CNTS) Solid Phase Microextraction (SPME) Probe and Application on the Analysis of Octylphenol and Nonylphenol in Water Coupled with Gas Chromatography-Mass Spectroscopy (GC-MS)
p.310

QSAR Studies on some of Dichloroacetyl Oxazolidine Herbicide Safeners
p.316

CoMFA Study on Novel Substitute Oxazolidine Derivatives
p.320

The Hydrogen Production of Mixed Acid Fermentation in Bio-Hydrogen Producing Reactor
p.326

Novel Approach to Fabricate an Amperometric Biosensor for Glucose Based on Nafion, Azure І and Gold Nanoparticles
p.331

Synthesis and Self-Assembly Behavior of Chiral Amphiphilic Diblock Copolymers Bearing L-Phenylalanine
p.334

Numerical Simulation of Inversion Population Distribution in Diode-End-Pumped Quasi-Three-Level Laser
p.338

[D-Ala2, D-Leu5]-Enkephalin (Dadle) Reduces Inflammation Responses after Transient Global Ischemia
p.343

Preparation, Characterization, and In Vitro Evaluation of Resveratrol-Loaded Bovine Serum Album Nanoparticles
p.349

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 345Novel Approach to Fabricate an Amperometric...

Novel Approach to Fabricate an Amperometric Biosensor for Glucose Based on Nafion, Azure І and Gold Nanoparticles

Abstract:

A novel approach to fabricate an amperometric biosensor for the detection of glucose was described. By means of self-assembled technique and the opposite-charged adsorption, Nafion(Naf), Azure І (Azu), gold nanoparticles (nano-Au) and glucose oxidase (GOD) were immobilized on the gold electrode subsequently. The GOD immobilized on the nano-Au monolayer was stable and retained its functional activity. The stepwise self-assemble procedure of the immunosensor was further characterized by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The factors influencing the performance of the resulting biosensor were studied in detail. The biosensor could detect the glucose in a range of 5.1×10-6~4.0×10-3mol/L.

You might also be interested in these eBooks

Smart Materials and Nanotechnology in Engineering

View Preview

Info:

Periodical:

Advanced Materials Research (Volume 345)

Pages:

331-333

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.345.331

Citation:

Cite this paper

Online since:

September 2011

Authors:

Yong Zhou

Keywords:

Azure І (Azu), Cyclic Voltammetry (CV), Glucose Biosensor, Gold Nanoparticles (Nano-Au)

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Upvan N, Paras N P, Frank V B. Glucose biosensor based on a Sol-Gel-Derived platform [J]. Anal. Chem., 1994, 66: 3139-3144.

Google Scholar

[2] Updike S J, Hicks G P. Enzyme electrode [J]. Nature, 1967, 214: 986-988.

DOI: 10.1038/214986a0

Google Scholar

[3] Pandey P C, Upadhyay S. Bioelectrochemistry of glucose oxidase immobilized on ferrocene encapsulated ormosil modified electrode [J]. Sensors and Actuators B, 2001, 76: 193-198.

DOI: 10.1016/s0925-4005(01)00632-3

Google Scholar

[4] Erica S. Forzani, Velia M. Sols, Ernesto J. Calvo. Electrochemical Behavior of Polyphenol Oxidase Immobilized in Self-Assembled Structures Layer by Layer with Cationic Polyallylamine[J]. Anal. Chem, 2000, 72 (21): 5300-5307.

DOI: 10.1021/ac0003798

Google Scholar

[5] Jianbo Jia, Bingquan Wang, Aiguo Wu, et a1. A method to construct a third generation horse radishperoxidase biosensor: Self-assembinggold nanoparticlesto three-dimensional solgel network [J]. Anal. Chem., 2002, 74 (9): 2217-2223.

DOI: 10.1021/ac011116w

Google Scholar