Preparation, Characterization, and In Vitro Evaluation of Resveratrol-Loaded Bovine Serum Album Nanoparticles

Abstract:

Resveratrol (RES) is a naturally occurring triphenolic phytoalexin compound exerting numerous beneficial effects in the organism. It has a wide range of biological activities in vitro as well as in vivo, such as anti-cancer, antioxidant, anti-inflammatory and beneficial cardiovascular effects. But, its low solubility in water led to its poor absorption in vivo and low bioavailability. Bovine serum album (BSA) nanoparticles have emerged as versatile desired carrier systems due to its ready availability, biodegradability, lack of toxicity and immunogenicity with fast development of nano technology. In this study, RES-BSANPS were prepared by a desolvation method and chemical cross-linking with glutaraldehyde successfully. Results controlled conditions (concentration of BSA, 10 mg/ml; pH = 9.0; volume of ethanol, 6 ml; volume of 0.25 % glutaraldehyde, 100 µl; amount of RES, 6.7 mg; cross-linking time, 24 h at room temperature (1 ml/min)) for entrapment efficiency, loading efficiency, mean particle size and zeta potential, were found to be 88.7 %, 39.4 %, 175.4 ± 0.5 nm, -35.93 ± 0.79 mV, respectively.

• Periodical: Advanced Materials Research (Volume 345)
• Pages: 349-354
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.345.349
• Online since: September 2011
• Authors: Jia Lei Li, Yuan Gang Zu, Xiu Hua Zhao, Dong Mei Zhao, Xiao Qiang Chen, Zhi Gang An, Kun Lun Wang
• Keywords: Bovine Serum Album (BSA), Characterization, In Vitro Evaluation, Nanoparticle, Resveratrol (RES)

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