Medium Optimization for the Antibacterial Substances Production from Brevibacillus brevis XDH Using Response Surface Methodology

Abstract:

Brevibacillus Brevis XDH is an antagonistic bacterium with high antibacterial activity against many pathogens. Fermentation medium for the production of the antibacterial substances was statistically optimized by Plackett-Burman design, steepest ascent experiments and response surface methodology. The optimized medium was composed of (g/L): glucose 15.6, soybean meal 28.4, starch 6, CaCO₃ 2 and MgSO₄ 4. After optimization, the production of antibacterial metabolites increased more than 70% than that of control.

• Periodical: Advanced Materials Research (Volume 345)
• Pages: 355-360
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.345.355
• Online since: September 2011
• Authors: Zhen Song, Xun Li Liu, Chang Xu Lu, Jian Yu, Rui Cheng Ju, Yu Hua Zhao, Jin Yu Li, Kai Qi Liu
• Keywords: Brevibacillus brevis, Medium Optimization, Response Surface Method (RSM)

References

[1] S. Li, Z. Zhao, M. Li, Z. Gu, C. Bai and W. Huang, Purification and Characterization of a Novel Chitinase from Bacillus brevis,  Acta Boic. Biop. Sinica, Vol. 34, pp.690-696, (2002).

[2] S.G. Edwards and B. Seddon, Mode of antagonism of Brevibacillus brevis against Botrytis cinerea in vitro, J. Appl. Microbiol. London, Vol. 91, pp.652-659, (2001).

DOI: 10.1046/j.1365-2672.2001.01430.x

[3] W. M. Haggag, Isolation of bioactive antibiotic peptides from Bacillus brevis and Bacillus polymyxa against Botrytis grey mould in strawberry, Arch. Phytopathol. Pflanzensch. London, Vol. 41, pp.477-491, (2008).

DOI: 10.1080/03235400600833704

[4] S. Chandel, E. J. Allan and S. Woodward, Biological Control of Fusarium oxysporum f. sp. lycopersici on Tomato by Brevibacillus brevis, J. Phytopathol. New York, Blackwell Verlag GmbH, (2009).

DOI: 10.1111/j.1439-0434.2009.01635.x

[5] Z. Wang and X. Liu, Medium optimization for antifungal active substances production from a newly isolated Paenibacillus sp. using response surface methodology, Bioresource Technol. London, Vol. 99, pp.8245-8251, (2008).

DOI: 10.1016/j.biortech.2008.03.039

[6] J. Su, Q. Zhou, H. Zhang, Y. Li, X. Huang and Y. Xu, Medium optimization for phenazine-1-carboxylic acid production by a gacA-qscR double mutant of Pseudomonas sp. M18 using response surface methodology, Bioresource Technol. London, Vol. 101, pp.4089-4095, (2010).

DOI: 10.1016/j.biortech.2009.12.143

[7] D. Xue, Identification of Brevibacillus Brevis XDH and isolation and characterization of its antibacterial substances, " Master, s thesis，Shandong Agricultural University, China, (2006).

[8] K. Chen, D. Xue, S. Xia, L. He, Z. Wang and X. Liu, Studies on formula of fermentation medium of strain Brevibacillus Brevis XDH, J. Shandong Agr. Univ. (Nat. Sci. ), Taian, Vol. 37, pp.190-195, (2006).

[9] X. Liu, G. He and Q. Chen, Culture medium optimization for producing cellulase by Trichoderma koningii ZJ5, J. Zhejiang Univ. (Eng. Sci. ), Hangzhou, Vol. 37, pp.623-628, (2003).

[10] R. Rahulana, K. M. Nampoothiri, G. Szakacsb, V. Nagyb and A. Pandeya, Statistical optimization of l-leucine amino peptidase production from Streptomyces gedanensis IFO 13427 under submerged fermentation using response surface methodology, Biochem. Eng. J. Switzerland, Vol. 43, pp.64-71, (2009).

DOI: 10.1016/j.bej.2008.08.011