The Study on the Interaction Conditions of Water-Soluble Chitosan with Bovine Serum Albumin

Abstract:

The interaction conditions between the water-soluble chitosans(WSC) and bovine serum albumin (BSA) was studied by Ultraviolet-visible absorption and fluorescence spectrometries. It was shown there was a good linear relationship between the absorbency A and the BSA concentration(0~1.5g/L) and WSC concentration (0~1.5 g/L). The fluorescence quenching of WSC to BSA was static quenching. When the temperature is 30°C, its binding constant Ka=5.35×104 L/mol, binding site n=1.05. The influence of WSC on the conformation of BSA was analyzed by sychronous fluorescence spectra and three-dimensional fluorescence spectrum.

• Periodical: Advanced Materials Research (Volumes 347-353)
• Pages: 1281-1286
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.347-353.1281
• Online since: October 2011
• Authors: Shan Shan Huang, Feng Zuo Qu, Tong Kuan Xu, Li Cui
• Keywords: Bovine Serum Albumin (BSA), Three-Dimensional Fluorescence Spectrum, Ultraviolet Spectrum, Water-Soluble Chitosans

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