Construction and Evaluation of Recombinant Strains for Ethanol Production from Lignocellulosic Hydrolysate

Shao Lan Zou; Chao Zhang; Yuan Yuan Ma; Le You; Min Hua Zhang

doi:10.4028/www.scientific.net/AMR.347-353.48

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 347-353Construction and Evaluation of Recombinant Strains...

Construction and Evaluation of Recombinant Strains for Ethanol Production from Lignocellulosic Hydrolysate

Abstract:

The recombinant Z.mobilis CX was constructed. Its ethanol concentration and ethanol yield from 2% xylose at 36 h were 6.73 g/L and 82.3% of theoretical yield, respectively. The recombinant S.cerevisiae YB was constructed and was showed to utilize cellobiose as the sole carbon source for rapid growth and ethanol production. The maximum ethanol concentration 7.493 g/L and ethanol yield 77.4% of theoretical yield from 2% cellobiose were obtained at 24 h. Further, the preliminary results of SSF of pretreated corn straw demonstrated the potential of improving ethanol production and reducing the costs of cellose enzymes used by co-fermentation of CX and YB.

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Advanced Materials Research (Volumes 347-353)

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48-51

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.347-353.48

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Online since:

October 2011

Authors:

Shao Lan Zou, Chao Zhang, Yuan Yuan Ma, Le You, Min Hua Zhang

Keywords:

Cellobiose Utilization, Ethanol, Lignocellulose, Saccharomyces cerevisiae, Xylose Utilization, Zymomonas Mobilis

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