Precision Cultivation of Phaeodactylum tricornutum and Free Lipid Acid Analysis

Abstract:

Abstract：A field test was conducted on a photobioreactors for cultivaion of P. tricornutum for optimization cultivation conditions.Here,we use a flat-cuvette pohotobioreactor to control the irradiance, pH, gas composition combined with on-line monitoring by fluorometer and densitometer.By chosing the culture medium in containing glucose，sodium acetate and glycerol, to check for the biomass, cell concentration, biochemical substances and fatty acid content nearly three months . The results show that: Phaeodactylum tricornutum is not only photoautotroph but also mixotroph, which with selectivity for substance concentration and organic carbon sources, the optimum concentration of glucose is 20mmol/L, the optimal growth condition in 500mL flask contains that the temperature is 25±1°C, the light intensity is 50µmol/m2.s, the pH is 8.5. Whatever the carbon sources are able to promote the biomass accumulation. When the cell concentration achieve to 2.5×106 cells/mL in the end of the culture, analyzed total lipid content so as to determine the biomass accumulation and biomass variation in different growth conditions. By ultrasonic extracting and freeze drying, the total of lipid reaches 20%(dry weight); the main content of fatty acid is C16:0,C18:0,which is the better material for biodisel production.

• Periodical: Advanced Materials Research (Volumes 354-355)
• Pages: 226-230
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.354-355.226
• Online since: October 2011
• Authors: Feng He, Peng Cheng Fu, Chun Ming Xu
• Keywords: Bio-Diesel, Biomass, Glucose, Light/Darkness, Organic Carbon Source, Phaeodactylum tricornutum, Total Liquid

References

[1] M.C. Cer´on Garc´ıa, J.M. Fern´andez Sevilla, F.G. Mixotrophic growth of Phaeodactylum tricornutum on glycerol: growth rate and fatty acid profile[J]. Journal of Applied Phycology 2000. 12: 239–248

[2] Sheehan J，Dunahay T，Benemaxm J，RoesslerP. A Look Baek at the U.S. Department of Energy，Biodiesel from Algae[M].The National Renewable Energy Laboratory(NREL)，Colorado.1998.

[3] Inna K G，Chiara B，Pushkar S，ZviC.Nitrogen starvation induces the Accumulation of arachidroic acid in the fresh water green alga Parietochloris Incis [J.]J Phycol，2002，38:991-994.

[4] Becker E W. Micro-algae as a source of protein[J]. Biotechnology Advances,2007, 25: 207-210.

[5] Gouveia L, Batista A P, Miranda A, et al. . Chlorella vulgaris biomass used as colouring source in traditional butter cookies[J]. Innovative Food Science and Emerging Technologies,2007, 8: 433-436.

DOI: 10.1016/j.ifset.2007.03.026

[6] Martinez-Fernandez E, Southgate P C. 2007. Use of tropical microalgae as food for larvae of the black-lip pearl oyster Pinctada margaritifera[J]. Aquaculture, 263:220-226.

DOI: 10.1016/j.aquaculture.2006.09.040

[7] Raja R, Hemaiswarya S, Rengasamy R. Exploitation of Dunaliella for β-carotene production[J]. Applied Microbiology and Biotechnology, 2007,74: 517-523.

DOI: 10.1007/s00253-006-0777-8

[8] Chisti Y. Biodiesel from microalgae[J].Biotechnology Advances, 2007,25: 294-306.

DOI: 10.1016/j.biotechadv.2007.02.001

[9] Huang K, Huang Z, Lv S H. Removal effects of microalga(Spirulina platensis) on landfill leachate purification[J]. Ecology and Environment,2006,15: 509-512.

[10] Milner H W，Lawrence N S，French C S.Colloidal dispersal of chloroplast material. Science,1950,111:633-634.

DOI: 10.1126/science.111.2893.633

[11] Shi J, Pan K H. Effects of different light intensities on growth and biochemical composition of Nitzschiaclosterium f. minutissima and Isochrysis galbana Parke8701[J]. Journal of Fishery Sciences of China,2004,11: 121-128.

[12] Abdullahi A S, Underwood G J C, Gretz M R.Extracellular matrix assembly in diatoms (bacillariophyceae) . V. Environmental effects on polysaccharide synthesis in the model diatom, Phaeodactylum tricornutum[J]. Journal ofPhycology,2006, 42: 363-378.

DOI: 10.1111/j.1529-8817.2006.00193.x

[13] Harrison P J, Waters R E, Taylor F J R. A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton[J]. Journal of Phycology, 1980,16: 28-35.

DOI: 10.1111/j.1529-8817.1980.tb00724.x