Optimizing Immobilization of Horseradish Peroxidase in the Nano-Scale Porous Structural Inorganic Material

Jung A Kim; Ji Sook Lim; Han Seung Kim

doi:10.4028/www.scientific.net/AMR.356-360.1085

Paper Titles

Degradation Kinetics and Mechanics of Methylene Blue by Complex Ultraviolet and Hydrogen Peroxide Process
p.1066

Catalytic CO₂ Reforming of Methane over Perovskite Noble Metals
p.1070

Cadmium Stress on Wheat Morphology: Germination and Growth
p.1075

A Novel PIV Interrogation Algorithm
p.1079

Optimizing Immobilization of Horseradish Peroxidase in the Nano-Scale Porous Structural Inorganic Material
p.1085

Decomposition of Chlorinated Hydrocarbons Using the Biocatalyst Immobilized by Clay Minerals
p.1089

Removal of Chromium(VI) by Zero Valent Iron: Effects of Environmental Factors
p.1093

Influence of Boiling Time on Purifying Sugar from Preserved Fruits Candied Liquid with Evaporation Method
p.1097

Treatment of Preserved Wastewater with Activated Sludge Method
p.1101

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 356-360Optimizing Immobilization of Horseradish...

Optimizing Immobilization of Horseradish Peroxidase in the Nano-Scale Porous Structural Inorganic Material

Abstract:

Horseradish peroxidase was immobilized by montmorillonite to construct a nano-scale structural biocatalyst for the stabilization and/or detoxification of phenolic compounds from natural waters. Humic substances extracted from soils were used as a bonding agent, stimulating peptide covalent bonds between enzymes and surface of inorganic clay minerals. Operating conditions including concentrations of enzyme inoculated and duration time and temperature for the immobilization were examined and the optimal condition for maximizing the quantity of enzyme immobilized was found. The results of this study are expected to provide the essential information for the process-efficient and economic enzyme immobilization operations in the environmental engineering as well as for establishing ecological green U-City.

You might also be interested in these eBooks

Progress in Environmental Science and Engineering (ICEESD)

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 356-360)

Pages:

1085-1088

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.356-360.1085

Citation:

Cite this paper

Online since:

October 2011

Authors:

Jung A Kim, Ji Sook Lim, Han Seung Kim

Keywords:

Enzyme Immobilization, Horseradish Peroxidase (HRP), Montmorillonite, Phenol

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] J.L. Gomez, A. Bodalo, E. Gomez, J. Bastida, A.M. Hidalgo, and M. Gomez, Immobilization of peroxidases on glass beads: An improved alternative for phenol removal. Enzyme and Microbial Technology, vol. 39 (2006), pp.1016-1022.

DOI: 10.1016/j.enzmictec.2006.02.008

Google Scholar

[2] V.A. Cooper and J.A. Nicell, Removal of phenols from a foundry wastewater using horseradish peroxidase. Water Research, vol. 30 (1996), pp.954-964.

DOI: 10.1016/0043-1354(95)00237-5

Google Scholar

[3] Y.C. Lai and S.C. Lin, Application of immobilized horseradish peroxidase for the removal of p-chlorophenol from aqueous solution, Process Biochemistry, vol. 40 (2005), pp.1167-1174.

DOI: 10.1016/j.procbio.2004.04.009

Google Scholar

[4] A.M. Klibanov, T.M. Tu and K.P. Scott, Peroxidase-catalyzed removal of phenols from coal-conversion waste waters, Science, vol. 221 (1983), pp.259-261.

DOI: 10.1126/science.221.4607.259

Google Scholar

[5] P. Grunw, Biocatalysis-biochemical fundamentals and applications, Imperial College Press, London (2009).

Google Scholar

[6] M. Monier, D.M. Ayad, Y. Wei and A.A. Sarhan, Immobilization of horseradish peroxidase on modified chitosan beads, International Journal of Biological Macromolecules. vol. 46 (2010), pp.324-330.

DOI: 10.1016/j.ijbiomac.2009.12.018

Google Scholar