Optimizing Immobilization of Horseradish Peroxidase in the Nano-Scale Porous Structural Inorganic Material

Abstract:

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Horseradish peroxidase was immobilized by montmorillonite to construct a nano-scale structural biocatalyst for the stabilization and/or detoxification of phenolic compounds from natural waters. Humic substances extracted from soils were used as a bonding agent, stimulating peptide covalent bonds between enzymes and surface of inorganic clay minerals. Operating conditions including concentrations of enzyme inoculated and duration time and temperature for the immobilization were examined and the optimal condition for maximizing the quantity of enzyme immobilized was found. The results of this study are expected to provide the essential information for the process-efficient and economic enzyme immobilization operations in the environmental engineering as well as for establishing ecological green U-City.

Info:

Periodical:

Advanced Materials Research (Volumes 356-360)

Edited by:

Hexing Li, Qunjie Xu and Daquan Zhang

Pages:

1085-1088

DOI:

10.4028/www.scientific.net/AMR.356-360.1085

Citation:

J. A. Kim et al., "Optimizing Immobilization of Horseradish Peroxidase in the Nano-Scale Porous Structural Inorganic Material", Advanced Materials Research, Vols. 356-360, pp. 1085-1088, 2012

Online since:

October 2011

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Price:

$35.00

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