Nickel Induced Oxidative Stress and the Responses of SOD Isoenzymes in Microcystis aeruginosa FACHB-905

Abstract:

The laboratory experiment was conducted to investigated the effect of nickel over a concentration gradient of 0.1~1.00 mg/L on biomass ( indicated by absorbance of cell culture at 663nm wavelength ), superoxide anion (O₂•^ˉ), malondialdehyde (MDA), and superoxide dismutase (SOD) in the cyanobacterium Microcystis aeruginosa FACHB 905 isolated from Dianchi Lake, south west of China . The results showed: (1) M. aeruginosa could live normally in Ni (II)-absence culture medium，but 0.3 mg/L Ni(II) was the fittest concentration for test M. aeruginosa, in which biomass and SOD activity were highest among all test cultures; (2) Ni(II) induced O₂•^ˉ and MDA in M. aeruginosa cells under the experimental condition, showing that Ni(II) could influence on M. aeruginosa by inducing oxidative stress; (3) Fe-SOD and Mn-SOD were found in M. aeruginosa and both of them were induced by lower Ni(II) but inhibited by higher concentration. The multiformity of SOD isoenzymes enhance the resistance of M. aeruginosa to oxygen stress induced by unfavorable condition, which explained that M. aeruginosa is the preponderant species in badly polluted Dianchi Lake water for long period.

• Periodical: Advanced Materials Research (Volumes 356-360)
• Pages: 119-126
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.356-360.119
• Online since: October 2011
• Authors: Li Juan Huang, Xue Xiu Chang, Cheng Wu
• Keywords: Dianchi Lake, Isoenzyme, Malondialdehyde (MDA), Microcystis aeruginosa, Nickel, Reactive Oxygen Species (ROS), Superoxide Dismutase (SOD)

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