The uses of herbicides have become an integral part of modern agricultural systems. The intensive use of herbicides atrazine has resulted in serious environmental problems. We researched the microbial Remediation of Atrazine-contaminated soil by Rhizobium sp. DNS7 and Acinetobacter sp DNS32. The results found two strains had a high capability for degrading atrazine in soil and culture medium. The mixed strains was more efﬁcient in the mineralization of atrazine than the isolated strain in soil. The result provided a effective way to remediate atrazine pollution soil.