Effect of Supplemental Fructus Ligustri Lucidi Extract on Methane Production and Rumen Fermentation in Sheep
The effect of addition of Fructus Ligustri Lucidi Extract (FLLE) on methane production and rumen fermentation in sheep was investigated in this study. FLLE addition levels were 0, 1, 2, 3 and 4 g/L of ruminal fluid. Ruminal fluid was removed from four sheep, mixed with phosphate buffer (1:2) and incubated (30 ml) anaerobically at 38°C for 24-h with or without FLLE. Results indicated that addition of FLLE linearly decreased methane production (p<0.05), linearly decreased ammonia concentration (p<0.05), and acetate to propionate ratio (p<0.05). Data of numbers of methanogens, fungi, ciliate and cellulolytic indicated that they were linearly decreased by addition of FLLE (p<0.05). Addition of FLLE at all dosages have no effect on rumen pH value, acetate molar concentration and butyrate molar concentration (p>0.05). Addition of FLLE linearly decreased count of F. succinogenes (p<0.05). In conclusion, these results of 24-h in vitro batch culture indicated that FLLE has the ability to inhibit methane production in the rumen, stimulate rumen fermentation and suppress fiber degrading bacteria, F. succinogenes. Abbreviations: FLLE=Fructus Ligustri Lucidi extract; IVDDM=in vitro digestibility of dry matter.
Hexing Li, Qunjie Xu and Daquan Zhang
G. H. Qiao et al., "Effect of Supplemental Fructus Ligustri Lucidi Extract on Methane Production and Rumen Fermentation in Sheep", Advanced Materials Research, Vols. 356-360, pp. 1454-1460, 2012