Effects of Si⁺, K⁺ and Ca²⁺ on Antioxidant Enzyme Activities and Osmolytes in Halocnemum strobilaceum under Salt Stress

Abstract:

We evaluated the effects of silicon , potassium, and calcium on the salt-tolerant plant Halocnemum strobilaceum (Pal.l) Bie under salt stress. The experimental treatments consisted of a NaCl-only treatment (150, 450, or 900 mmol/l NaCl), a complex salts treatment (NaCl with K⁺, Ca²⁺ ,andSi⁺at total concentrations of 150, 450, or 900 mmol/l;(Na⁺:K⁺:Ca²⁺ :Si⁺ =1:0.03:0.14:0.004), and a control with no complex ions or NaCl. After 20 and 60 days of treatments, we investigated activities of the major antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), as well as succulence and the contents of malondialdehyde (MDA), proline(Pro) and glycine betain (GB). We found that additions of K⁺ , Ca²⁺ , and Si⁺ partially alleviated the negative effects of salinity stress by increasing the salt tolerance of the plant. The improved salt tolerance was associated with increased Pro, GB,and increased activities of SOD, CAT, and POD,and decrease MDA. In contrast, the NaCl-only treatments caused marked decreases in succulence and soluble protein contents. The results of these experiments suggest that K⁺, Ca²⁺ , and Si⁺ can alleviate the damaging effects of salt on H. strobilaceum by preventing oxidative membrane and oxidant enzyme activities damage, and that they may be associated with osmotic adjustment.

• Periodical: Advanced Materials Research (Volumes 356-360)
• Pages: 2542-2550
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.356-360.2542
• Online since: October 2011
• Authors: Dan Su, Nian Lai Chen, Tian Peng Gao, Chun Yan Wang, Hong Mei Sheng, Cai Ling Yang
• Keywords: Osmotic Adjustment, Oxidative Stress, Potassium and Calcium, Salt Stress, Silicon

References

[1] Viswanathan C,Andreamd J,Zhu JK.Crop Sci Vol.45(2005),p:437-448

[2] Jungs.Plant Science Vol.166(2004),P.459-466

[3] Mccord T.M. American Journal of Medicine.Vol.108(2000),P.652-659

[4] Smirnoff N.New Phytologist.Vol.(125),P.27-58

[5] MeloniDA.Oliva MA,MartinezCA,Cambraia J.Environ Exp Bot.Vol.(49),P.69-76

[6] Sudhakar A,Lakshmi S,Giridarakumar A.Plant Science.Vol.161(2001),P.613-619

[7] Mittler.Trends in Plant Science.Vol.(7),P.405-410

[8] Liu T,Staden JV.Plant Growth Rej.Vol.(34),P.277-285

[9] Clatcssen w.Plant Sci.Vol.(168),P.241-248

[10] Epstein E. Plant Physilolgy.Vol.(50),P.641-664

[11] Epstein E..Prioc Natl Acad Sci USA.Vol.(91),P.11-17

[12] Gong H.Z. Chen K.,Wang S,Zhang C. Plant Science.Vol.169(2005),P.313-321

[13] LiQF,MaCC,ShangQL.Chinese Jouranal of Applied Ecology.Vol.(18),P.531-536

[14] Baohe Miao, Xing Guo Han,Wen hao Zhang.Annals of Botany.Vol.105(2010),P.967-973.

[15] Qi Z,Spalding EP.Plant Physiology.Vol.136(2004),P.2547-2555

[16] Lieberbach H,Steingrobe B,Classen N.Plant Soil.Vol.260(2004),P.79-88

[17] Wei HU,Jing zhao LI,Shu xiang ZHANG.Chinese Journal of Eco-Agriculture.Jan.Vol.19(2011),P.98-102.

[18] Mcainsh P R, Brownlee AM, Hetherington AM. Physiologia Plantarum.Vol.100(1997),P.16-21

[19] Bing Han,Lie Sun.Acta Horticulturae sinica.VOL.37(2010 ),P.1937-1943.

[20] Hoagland DR,Arnon DS Calif.Agric.Exp.Stat.Circ.VOL.34(1950),P.3471-3472.

[21] SudhakarC, Lakshmi A, Giridarakumar S .Plant Sci.Vol.161(2001),P.613–619

[22] Giannopolitis CN, Ries SK. Plant Physiology.VOL.59(1977),P.309–314

[23] Liang YC, Chen Q, Zhang WH, Ding RX. Plant Physiology.Vol.160(2003):,P.1157–1167.

[24] BatesL.S. WaldrenR.P. TeareI.D. Journal of Plant Soil.VOL.39(1973),P.205–217.

[25] Grieve C.M., Grattan S.R. Plant and Soil.VOL.70(1983): P.303–307.

[26] MA J.E,MZYAKEY,TAKAYASHZ(2001)In:DATHOFF C.E, SNYUEKG.H

[27] Ke fu Zhao,Hai Fan.Saline plants and adapt to the physiological saline habitat.Beijing, Science Press,111-115(2005)

[28] Flowers TJ, Hajibagheri MA,Clipson NJW,Haophytes(1986).The Quarterly Review of Bilology.VOL.61(1986),P.313-317.

[29] MomonokiYS,KaminuraH.Japanese Journal of Crop Science.Vol.63(1994),P.518-523

[30] Apel k,HintY 2004: Plant Bive.Vol.55(2004),P.373-399.

[31] GomezJM,Her nandezJA,JimemezA,del RioCA,sevillaF.Free Radic Rse.Vol.31(1999),Ps11-s18

[32] MittovaV,Talm,Volokita M,Couy M.plant cell Envtoon.Vol.26(2003),P.845-856

[33] T,J ftowers.Tournal of experymant Botany .Vol.396(2003),P.307-319

[34] Munns R,Testor M. Annu.Rev.Plant Biol.VOL.59(2008),P.651-681.

[35] Ashraf M,Harris PJC.Plants Sci1.Vol.66(2004),P.3-16

[36] LiangYC,ZhuJ,LiZj:Enironmental and Experimental Botany.Vol.64(2008),P.286-294.

[37] Rengel Z,Damwn PM.physiology plantarum.Vol.133(2008),P.624-636.

[38] Nakano Y,Asada k Plant cell physiel.Vol.22(1981),P.867-880

[39] Noctor G,Veljvoic-Jovanovic s,Foyer c.y.Philosophocal Transcations of the Koyal society of london.VOL.355(2000),P.1465-1475

[40] Genardy,SaosJ.L.E,Htcgaod J,Tremdienes A,BoucudJ.plant physidogy and Biochemisry,Vol.29(1991),P.421-427

[41] DelauneyAJ,Averma DPS.plant Journal.Vol.5(1993),P.215-223