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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 356-360Fast Degradation of Chlorpyrifos in Soil by Strain...

Fast Degradation of Chlorpyrifos in Soil by Strain Cupriavidus taiwanensis X1 Labeled with LuxAB

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Abstract:

For researching ecological behaviors of Cupriavidus taiwanensis X1 which has strong hydrolysis activity on chlorpyrifos(CP), reporter gene luxAB was successfully introduced into cells by electroporation. The labeled strain X1-lux with genetic stability and fluorescence was obtained. Both of strain X1 and X1-lux could completely degrade 200 mg/l CP within 12h in minimal salt medium, and experimental results showed that introduction of luxAB did not affect strain X1 growth and degradation on CP. The cells of X1-lux and X1 were inoculated into soil with 500 mg/l CP, the cell concentration and CP residual was detected, and data revealed that strain X1 could absolutely removed CP in 12d and survive in soil. Strain X1 is a potential excellent choice for bioremediation of organophosphorus polluted environments.

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Progress in Environmental Science and Engineering (ICEESD)

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Periodical:

Advanced Materials Research (Volumes 356-360)

Pages:

2566-2570

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.356-360.2566

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Online since:

October 2011

Authors:

Bei Bei Zhu, Dao Sheng Wang, Yuan Yuan Cao, Xin Yun Tang, Ri Mao Hua, Tao Zhong Shi

Keywords:

Chlorpyrifos, Cupriavidus Taiwanensis, Degradation, Electroporation, LuxAB, Soil

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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