Ferrooxidans’s Isolation and Purification

Abstract:

we take mining water from Liang Zou group undergroud 200 m. and cltured in 9K medium, sodium thiosulfate medium, sulfur (S⁰) medium. According to its conformation on the different mediums, we get ferrooxidans(T.f), and studying its ecological nature shows that the ferrooxidanis is acidophilic autotrophic bacteria and has ferrous iron and sulfur oxidation. Its optimum growth pH value is 2.0, optimum growth temperature is 30°C, 1.0 g/L of Fe^{2 +} and 1 % S0 which can be used as the energy source are propitious to its growth. After UV mutagenesis, the strain which has been cold treated improve Fe²⁺ oxidation ability, at the same time, shorten the time enter to the logarithmic growth phase, the density of bacteria in lag growth phase increases from 6.8×107 cells/mL to 7.8×107 cells/mL, so increase bio-leaching capability.

• Periodical: Advanced Materials Research (Volumes 356-360)
• Pages: 3-7
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.356-360.3
• Online since: October 2011
• Authors: Li Hua Lv, Jing Cheng Ren, Tian Hu Sun, Jian Qing Dai
• Keywords: Bioleaching, Thiobacillus ferrooxidans, UV-Mutagenesis

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