Ferrooxidans’s Isolation and Purification
we take mining water from Liang Zou group undergroud 200 m. and cltured in 9K medium, sodium thiosulfate medium, sulfur (S0) medium. According to its conformation on the different mediums, we get ferrooxidans(T.f), and studying its ecological nature shows that the ferrooxidanis is acidophilic autotrophic bacteria and has ferrous iron and sulfur oxidation. Its optimum growth pH value is 2.0, optimum growth temperature is 30°C, 1.0 g/L of Fe2 + and 1 % S0 which can be used as the energy source are propitious to its growth. After UV mutagenesis, the strain which has been cold treated improve Fe2+ oxidation ability, at the same time, shorten the time enter to the logarithmic growth phase, the density of bacteria in lag growth phase increases from 6.8×107 cells/mL to 7.8×107 cells/mL, so increase bio-leaching capability.
Hexing Li, Qunjie Xu and Daquan Zhang
L. H. Lv et al., "Ferrooxidans’s Isolation and Purification", Advanced Materials Research, Vols. 356-360, pp. 3-7, 2012