Kinetic Studies for the Inhibition Effect of Pentachlorophenol on Alkaline Phosphatase Activity In Vitro

Abstract:

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The inhibition kinetic parameters of pentachlorophenol (PCP) on bovine intestinal mucosa alkaline phosphatase (ALP) activity were studied by spectrophotometry. The results showed that PCP inhibited ALP activity in a concentration dependent manner, and the 50% inhibitory concentration (IC50) was estimated to be 4.20 mM. The apparent Michaelis–Menten constant (Km) and apparent maximum reaction rate (Vmax) was found to be decreased in the presence of PCP. Lineweaver–Burk plots indicated that the nature of the inhibition was of an uncompetitive type. Kinetic analysis indicated that the value of the inhibition constant (Ki) was 5.67 mM.

Info:

Periodical:

Advanced Materials Research (Volumes 356-360)

Edited by:

Hexing Li, Qunjie Xu and Daquan Zhang

Pages:

309-312

DOI:

10.4028/www.scientific.net/AMR.356-360.309

Citation:

Q. Y. Liu "Kinetic Studies for the Inhibition Effect of Pentachlorophenol on Alkaline Phosphatase Activity In Vitro", Advanced Materials Research, Vols. 356-360, pp. 309-312, 2012

Online since:

October 2011

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$35.00

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