Isolation and Characteristic of Chondroitin Sulfate from Grass Carp Scales

Shuang Li Xiong; An Lin Li; Min Juan Shi

doi:10.4028/www.scientific.net/AMR.391-392.1003

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 391-392Isolation and Characteristic of Chondroitin...

Isolation and Characteristic of Chondroitin Sulfate from Grass Carp Scales

Abstract:

Papain and alcalase were successively used to hydrolyze grass carp scales for preparation of crude polysaccharide. Chondroitin sulfate were further purified by DEAE-Sepharose Fast Flow and Sepharose 6 Fast Flow gel column chromatograph, and indentified by IR, NMR, element analysis and chemical analysis. Three polysaccharide fractions including chondroitin sulfate were fistly obtained from grass carp scales. The weight-average molecular weight of chondroitin sulfate was 46168 Da. The contents of various elements (C, H, N, S, O) in chondroitin sulfate were 28.89 %, 3.80 %, 3.36 %, 6.71 % and 47.16 %, respectively. The results of IR, NMR and chemical analysis exhibited that the sulfate group in chondroitin sulfate be O-linked in position 4 of N-acetyl-D-galactosamine.

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Advanced Materials Research (Volumes 391-392)

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1003-1007

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https://doi.org/10.4028/www.scientific.net/AMR.391-392.1003

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Online since:

December 2011

Authors:

Shuang Li Xiong, An Lin Li, Min Juan Shi

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Chondroitin Sulfate, Grass Carp, Identification, Isolation, Purification, Scale

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