To Enhance the Production of Human-Like Collagen II by Amino Acid Addition Using Response Surface Methodology

Abstract:

Amino acids, the basic unit of protein molecules, are closely related with biological activities. In order to enhance the expression of human-like collagen (HLC), amino acids were added into fermentation broth, and response surface methodology (RSM) to optimize the categories and the concentration of amino acids. The results of PB-design showed that only the dosage of alanine and glutamate had significant effects on the production of HLC. Quadratic polynomial models were established after analyzing the results of CCD-design. The optimal concentration of alanine and glutamate were 3.78gL-1、and 2.52g•L-1 respectively. Under the optimal cultivation condition the maximal production of human-like collagen could be up to 0.292g•L-1 which was 11.45% higher than that of the control group 0.262g•L-1 (the highest production of the former study).

• Periodical: Advanced Materials Research (Volumes 393-395)
• Pages: 1054-1059
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.393-395.1054
• Online since: November 2011
• Authors: Lei Guo, Yan E Luo, Dai Di Fan, Ru Xu
• Keywords: Amino Acids Addition, Human-Like Collagen (HLC), Response Surface Methodology (RSM)

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