The Role of Collagen Type I on Hematopoietic and Mesenchymal Stem Cells Expansion and Differentiation

Abstract:

The three dimensional scaffold of the bone marrow (BM) niches is composed of various elements including extracellular matrix proteins and cell types, such as collagen type I (Col I) and stroma cells. Interaction of stem cells with their microenvironment is important for their regulation. In the marrow, Col I is mostly localized in the endosteal regions. The objective of this work was to investigate the role of Col I in the regulation of Hematopoietic Stem Cells (HSC) and Mesenchymal Stem Cells (MSC) growth. Col I was extracted from rat tail tendons and its purity confirmed. Human BM MSCs and umbilical cord blood (UCB) CD34⁺ cells were used as Stem Cell sources. MSCs were cultured in medium with serum while CB CD34⁺ cells were cultured without serum with cytokines. The impact of increasing concentrations of Col I (0-50 µg mL^-1 for coating) on the growth of Hematopoietic Progenitor Cells (HPC) and MSCs was investigated by cytometry, microscopy and clonogenic progenitor assays. Only a minority of CD34⁺ cells expressed the Col I receptor α₂β₁ prior to culture, while the opposite was observed when hematopoietic cells were placed in culture. Col I coated surfaces reduced the expansion of hematopoietic cells by 25% compared to control, while expansions of myeloid and MK progenitors were either unchanged or negatively affected by Col I, respectively. The differentiation of HPCs was also affected on Col I as demonstrated by differences in the frequencies of various cell lineages, such as CD34⁺ cells, megakaryocytes (MK), erythrocytes and others. In contrast to HPCs, Col I surfaces increased MSCs proliferation but had little impact on osteoblasts derived from MSCs. Taken together, this study provides new insights into the regulatory activities of Col I on Stem Cells residing in the marrow.

• Periodical: Advanced Materials Research (Volume 409)
• Pages: 111-116
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.409.111
• Online since: November 2011
• Authors: Betül Çelebi, Nicolas Pineault, Diego Mantovani
• Keywords: Collagen Type I, Extracellular Matrix Protein, Hematopoietic Progenitor Cells

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