Ultrasonic Setup for Testing Hydrogels: Preliminary Experiments on Collagen Gels

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The assessment of mechanical properties of highly hydrated natural materials remains a challenge because, in general, their mechanical evaluation implies invasive and finally destructive methods. Acoustic-based tests may represent the appropriate tools to investigate the mechanical properties of such materials, particularly collagen gels, whose acoustic properties are poorly understood. The objective of this work is to develop two experimental setups for the assessment of acoustic properties of such a hydrogels. In the first one, a typical pulse echo reflectometer was implemented. The acoustic parameters were measured at controlled temperature in an especially designed chamber. In the second one, the previous configuration was combined with a setup for compressive tests, allowing to interrogate simultaneously both the acoustic and mechanical properties of the sample under test. The frequency of the acoustic transducer was 10MHz. The acoustic and mechanical properties of collagen gels prepared according to different experimental conditions (pH and collagen concentration) were evaluated. The first set of experiment was useful to accomplish estimation of the speed of sound, attenuation and acoustic impedance. The second one allowed us to monitor the speed of sound during the evolution of the compression test. This approach could be a potential tool to study the changes in hydrogels mass density and bulk compressibility.

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146-151

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November 2011

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] Niklason L. E., Gao J., Abbott W. M., Hirschi K. K., Houser S., Marini R. and Langer R. Functional Arteries Grown in Vitro, , Science 16 April 1999, Vol. 284 no. 5413 pp.489-493.

DOI: 10.1126/science.284.5413.489

Google Scholar

[2] Peter Fratzl (Ed) Collagen: Structure and Mechanics, Springer, 2008, XVIII, ISBN: 978-0-387-73905-2.

Google Scholar

[3] Achilli M. and Lagueux J. and Mantovani D., On the effects of UV-C and pH on the mechanical behavior, molecular conformation and cell viability of collagen-based scaffold for vascular tissue engineering, Macromolecular Bioscience, 307-316, 10, (2010).

DOI: 10.1002/mabi.200900248

Google Scholar

[4] Hall T. J., Bilgen M., Insana M. F. and Krouskop T. A., Phantom Materials for Elastography, IEEE trans. on ultrasonics, ferroelectrics, and frequency control, 44, 6, (1997).

DOI: 10.1109/58.656639

Google Scholar

[5] Zaucha M. T., Raykin J., Wan W., Gauvin R., Auger F. A., Germain L., Michaels, T. E. and Gleason R., A Novel Cylindrical Biaxial Computer-Controlled Bioreactor and Biomechanical Testing Device for Vascular Tissue Engineering, Tissue Engineering, 15, 11, 3331-3340 (2009).

DOI: 10.1089/ten.tea.2008.0369

Google Scholar

[6] Rice M. A., Waters K. R. and Ansetha K. S., Ultrasound monitoring of cartilaginous matrix evolution in degradable PEG hydrogels, Acta Biomaterialia, 5, 1, 152-161 (2009).

DOI: 10.1016/j.actbio.2008.07.036

Google Scholar

[7] Rajan N. and Habermehl J. Coté M.F. Doillon C.J. and Mantovani D., Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications, Nature Protocols, 2753-2758, 1, (2006).

DOI: 10.1038/nprot.2006.430

Google Scholar

[8] McClements D.J. and Fairley P., Ultrasonic pulse echo reflectometer, Ultrasonics, 58-62, 29, (1991).

DOI: 10.1016/0041-624x(91)90174-7

Google Scholar

[9] Insana M.F. and Hall T.J. and Chaturvedi P. and Kargel Ch., Ultrasonic properties of random media under uniaxial loading, Journal of the Acoustical Society of America, 3243-3251, 110, 6, (2001).

DOI: 10.1121/1.1414703

Google Scholar