Construction of Luciferase Reporter Plasmid with Human AQP1 Gene Promoter

Abstract:

Aquaporins (AQPs), a family of water channels, function as a water-selective transporting protein in cell membranes. Aquaporin 1 (AQP1) was first discovered in human erythrocytes as a water channel for high osmotic water permeability. AQP1 promoter is responsible for regulation of its transcription and expression. In this study, molecular biology and genetic engineering knowledge and principles of construction of AQP1 promoter plasmid, and recombinant plasmids were identified and measured for luciferase, for promoter function. Our study successfully constructed AQP1 promoter luciferase reporter gene expression plasmid and successfully carry out the functions of its detection, provides a new foundation for the transcription expression and function regulation of the AQP1

• Periodical: Advanced Materials Research (Volumes 424-425)
• Pages: 399-402
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.424-425.399
• Online since: January 2012
• Authors: Zhe Sun, Tong Cun Zhang, Yong Jiang
• Keywords: AQP1, MRTF-A, Promoter

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