Preparation of Artificial Antigen and Immunological Trait for Estradiol

Abstract:

This paper presents an indirect competitive enzyme-linked immunosorbent assay (icELISA) using anti-mouse polyclonal antibody for rapid, sensitive analysis of 17-beta-estradiol (E2) residue. After derivation with succinic anhydride, E2 was coupled to bovine serum albumin (BSA) and ovalbumin (OVA) through carbodiimide active ester (EDC) method, and the conjugation ratio of E2-BSA was 18.6:1. Using mouse anti-E2 polyclonal antibody, an icELISA standard curve was established. The optimal concentrations of the coated E2-OVA and anti-E2 pAb were 2 μg/mL, and 1:32 000 dilutions, respectively, by the checkerboard titration. This method was sensitive and had a linear range from 0.16 to 128 ng/mL, with IC50 and LOD values of 3.76 ng/mL and 0.08 ng/mL. Therefore, the established icELISA provides a useful screening method for quantitative or qualitative detection of E2 residue in tissues or liquids.

• Periodical: Advanced Materials Research (Volumes 424-425)
• Pages: 612-615
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.424-425.612
• Online since: January 2012
• Authors: Shi Xiu Wu, Jin Qing Jiang
• Keywords: Artificial Antigen, Characteristic, Estradiol, Synthesis

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