Construction of Reagentless Biosensor Based on Self-Assembly and Electrodeposition for Determination of Hydrogen Peroxide

Dong Jiao Zhao; Yan Hong Chen; Chen Di Tu; Yao Fang Xuan; Feng Na Xi

doi:10.4028/www.scientific.net/AMR.441.442

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 441Construction of Reagentless Biosensor Based on...

Construction of Reagentless Biosensor Based on Self-Assembly and Electrodeposition for Determination of Hydrogen Peroxide

Abstract:

A general methodology to prepare reagentless biosensor was developed based on self-assembly and electrodeposition. Redox active inorganic multilayers consisting of copper hexacyanoferrate (CuHCF) multilayers were formed by successive self-assembly. A simple and controllable electrodeposition approach was established for one-step fabrication of chitosan-enzyme layer on CuHCF modified electrode. Horseradish peroxidase was selected as the model enzyme. With CuHCF as the electroactive mediator, the developed reagentless biosensor exhibited a fast amperometric response for the determination of hydrogen peroxide (H₂O₂). The linear response ranged from 1.4 × 10^-5 to 2.0 × 10^-4 M with a detection limit of 1.2 × 10^-6 M. The biosensor exhibited high reproducibility and long-time storage stability. The proposed methodology could serve as a versatile platform for fabrication of electrochemical biosensors.

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Periodical:

Advanced Materials Research (Volume 441)

Pages:

442-446

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.441.442

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Online since:

January 2012

Authors:

Dong Jiao Zhao, Yan Hong Chen, Chen Di Tu, Yao Fang Xuan, Feng Na Xi

Keywords:

Biosensor, Electro-Deposition, Hydrogen Peroxide, Reagentless, Self-Assembly

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