Purification and Characterization of a Bioscouring Pectate Lyase from Paenibacillus sp. WZ008 with High Activity on Pectin

Xiang Xian Ying; Li Na Chen; Mei Lan Yu; Qun Xue; Zhao Wang

doi:10.4028/www.scientific.net/AMR.441.457

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 441Purification and Characterization of a Bioscouring...

Purification and Characterization of a Bioscouring Pectate Lyase from Paenibacillus sp. WZ008 with High Activity on Pectin

Abstract:

An extracellular pectate lyase was purified from the culture supernatant of Paenibacillus sp. WZ008 grown in the pectin-containing medium. The enzyme was purified to homogeneity in three steps and found to have a molecular weight of around 45 kDa. Highly methylated pectin was the optimum substrate in the case of no Ca²⁺ addition while the enzyme exhibited the maximal activity on polygalacturonic acid in the presence of 4 mM Ca²⁺. The purified enzyme demonstrated the optimum activity at a temperature range of 55-60°C and pH 9.6. The Ca²⁺ ion enhanced the enzyme activity but Mn²⁺, Ba²⁺and EDTA strongly inhibited it.

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Periodical:

Advanced Materials Research (Volume 441)

Pages:

457-461

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.441.457

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Online since:

January 2012

Authors:

Xiang Xian Ying, Li Na Chen, Mei Lan Yu, Qun Xue, Zhao Wang

Keywords:

Alkaline Pectate Lyase, Characterization, Purification

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