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Lipase Immobilization on Functional Group Controlled Surfaces

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Abstract:

A simple means was demonstrated to control the functional group on surface and use this surface as a model support to investigate the effect of surface chemistry on lipase adsorption and its activity. It was based on mixing polystyrene (PS) solution with small molecular weight functional group-terminated polystyrenes (PS-Fs, functional groups such as-CH3, -CF3, -NH2, -COOH and-OH) and casting them on glass plates to fabricate the functional group controlled surfaces. The amount of adsorbed lipase on the surface with similar functional group density was-CH3 > PS > -CF3 > -NH2 > -COOH > -OH. The activity retention of the immobilized lipase was-OH > -NH2 > -COOH > -CF3 > -CH3 > PS.

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Periodical:

Advanced Materials Research (Volume 441)

Pages:

452-456

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.441.452

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Online since:

January 2012

Authors:

Peng Ye, Xin Ping Wang, Zhu Ping Han, Jie Yang, Rong Bing Wan

Keywords:

Enzyme Immobilization, Functional Group Controlled Surfaces, Lipase, Surface Chemistry

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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