Nanobiocomposite-Based Bienzyme Screen-Printed Electrode for Glucose Monitoring

Abstract:

Multi-walled carbon nanotubes (MWNTs) were coated first with methylene blue (MB) by noncovalent adsorption and then by horseradish peroxidase (HRP) and glucose oxidase (GOD) by covalent-linking. The MB-MWNTs/GOD/HRP composites formed stable films on screen-printed electrodes (SPE). The electrochemical and electrocatalytic behaviors of the bienzyme modified SPE were studied using cyclic voltammetry (CV) and amperometry. Using the MB-MWNT/GOD/HRP composites, glucose could be calibrated by amperometry at −0.35 V vs. SCE. The optimized response (at pH 7.0) had a sensitivity of 2.4 mA.M−1 and a limit of detection (3×S/N) of 35 µM. Tests of the portable bienzyme sensor demonstrate its imperviousness to the effects of ascorbic acid, uric acid and p-acetaminophen, as well as its ability to measure glucose concentrations ranging from 0.25 to 2 mM.

• Periodical: Advanced Materials Research (Volumes 452-453)
• Pages: 909-912
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.452-453.909
• Online since: January 2012
• Authors: Saithip Pakapongpan, Surachai Thachepan, Yingyot Poo-Arporn, Rungtiva Palangsuntikul
• Keywords: Bienzyme, Carbon Nanotube (CN), Composite, GOD, HRP, Methylene Blue (MB)

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