Determination of Hydrogen Peroxide Residue in Food Using CdS Quantum Dots as Fluorescence Probes

Abstract:

A simple and accurate method for determination of hydrogen peroxide (H₂O₂) using carboxymethyl cellulose coated CdS quantum dots (QDs) as a fluorescence probe was established. The influence factors of the fluorescence quenching system and the optimum conditions were investigated for analysis of hydrogen peroxide. It was found that the maximum relative fluorescence quenching intensity produced at pH 8.5 in 0.035 mol/L KH₂PO₄-Na₂HPO₄, when the concentration of CdS QDs was 1.9×10-3 mol/L and the reacting time and temperature were 45 minutes and 30°C, respectively. Under the optimum conditions, the relative fluorescence quenching intensity has a linear relationship with the logarithmic concentration of H₂O₂ in the range from 3×10-5 to 5×10-2 mol/L. The limit of the detection is 2.3×10-6 mol/L for H₂O₂. The method was used to determinate the amount of remained H₂O₂ in milk and Calcium Tablets successfully.

• Periodical: Advanced Materials Research (Volumes 455-456)
• Pages: 1189-1194
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.455-456.1189
• Online since: January 2012
• Authors: Yun Long Zeng, Ya Jing Wang, Zhong Hua Su, Chun Ran Tang
• Keywords: Carboxymethyl Cellulose Coated CdS QDs, Fluorescence Quenching, Food, Health Food, Trace Hydrogen Peroxide

References

[1] O. S. Wolfbeis, A. Durkop, M. Wu and Z. H. Lin, A europium-ion based luminescent sensing probe for hydrogen peroxide, Angew. Chem. Int. Ed, vol. 41, no. 23, p.4495–4498, (2002).

DOI: 10.1002/1521-3773(20021202)41:23<4495::aid-anie4495>3.0.co;2-i

[2] K. A. Fahnrich, M. Prawda and G. G. Guilbault, Recent applications of electrogenerated chemiluminescence in chemical analysis, Talanta, vol. 54, no. 4, pp.531-559, (2001).

DOI: 10.1016/s0039-9140(01)00312-5

[3] M. BruchezJr, M. Moronne, P. Gin, S. Weiss and A. P. Alivisatos, Semiconductor nanocrystals as fluorescent biological labels, Science, vol. 281, no. 5385, pp.2013-2016, (1998).

DOI: 10.1126/science.281.5385.2013

[4] R. Yang and S. P. Liu, Development of some molecular spectral analytical methods for the determination of proteins, Chin. J. Anal. Chem., vol. 29, no. 2, pp.232-24129, (2001).

[5] H. Mattoussi, J. M. Manro, E. R. Goldman, G. P. Anderson, V. C. Sundar, F. V. Mikula, et al,. Self-assembly of CdSe−ZnS quantum dot bioconjugates using an engineered recombinant protein, J. Am. Chem. Soc., vol. 122, no. 49, pp.12142-12150 , (2000).

DOI: 10.1021/ja002535y

[6] C. W. Chan, S. M. Nie, Quantum dot bioconjugates for ultrasensitive nonisotopic detection, Science, vol. 281, pp.2016-2018, (1998).

DOI: 10.1126/science.281.5385.2016

[7] X. Michalet, F. F. Pinaud, L. A. Bentolila, J. M. Tsay, S. Doose, J. J. Li, et al., Quantum dots for live cells, in vivo imaging, and diagnostics, Sicence, vol. 307, no. 5709, pp.538-544, (2005).

DOI: 10.1126/science.1104274

[8] R. E. Bailey, A. M. Smith and S. Nie, Quantum dots in biology and medicine,  Physica E, vol. 25, no. 1, pp.1-12, (2004).

[9] X. Hu, H. Han, L. Hua, Z. Sheng, Electrogenerated chemilumine- scence of blue emitting ZnSe quantum dots and its biosensing for hydrogen peroxide, Biosens. Bioelectron., vol. 25, no. 7, pp.1843-1846, (2010).

DOI: 10.1016/j.bios.2009.12.021

[10] H. Han, Z. Sheng and J. Liang, Electrogenerated chemiluminescence from thiol-capped CdTe quantum dots and its sensing application in aqueous solution,. Anal. Chim. Acta, vol. 596, no. 1, pp.73-78, (2007).

DOI: 10.1016/j.aca.2007.05.039

[11] Z. Wang, Q. Xu, H.Q. Wang, Q. Yang, J. H. Yu, Y.D. Zhao, Hydrogen peroxide biosensor based on direct electron transfer of horseradish peroxidase with vapor deposited quantum dots,. Sens. Actuat. B, vol. 138, no. 1, pp.278-282, (2009).

DOI: 10.1016/j.snb.2008.12.040

[12] C. G. Shi, J. J. Xu and H. Y. Chen, Electrogenerated chemilumine- scence and electrochemical bi-functional sensors for H2O2 based on CdS nanocrystals/ hemoglobin multilayers, J. Electroanal. Chem., vol. 610, p.186–192, (2007).

DOI: 10.1016/j.jelechem.2007.07.018

[13] C. R. Tang, Z. H. Su, B. G. Lin, H.W. Huang, Y.L. Zeng, S. Li, H. Huang, Y. J. Wang, et al., A novel method for iodate determination using cadmium sulfide quantum dots as fluorescence probes,. Anal. Chim. Acta, vol. 678, pp.203-207, (2010).

DOI: 10.1016/j.aca.2010.08.034

[14] D. Pan, Q. Wang, S Jiang, X. Ji and L. An, Controllable synthesis of highly luminescent and monodisperse CdS nanocrystals by a two-phase approach under mild conditions, Advan. Mater., vol. 16, no. 12, pp.982-985, (2004).

DOI: 10.1002/adma.200400010

[15] B. R. Sankapal, R. S. Mane, C. D. Lokhande, Deposition of CdS thin films by the successive ionic layer adsorption and reaction (SILAR) method, Mater. Res. Bull., vol. 35, no. 2, pp.177-184, (2000).

DOI: 10.1016/s0025-5408(00)00210-5