Impacts of Uranyl Ion on the Structure and Function of Cytochrome b₅ His39Ser Mutant

Abstract:

Uranium is toxic to human body with mechanisms not fully understood. The structural and functional consequences of uranyl ions (UO₂²⁺) interacting with an axial mutant of cytochrome b₅, His39Ser (cyt b₅ H39S), were investigated by both spectroscopic and molecular modeling methods. Although slightly disturbs protein folding, UO₂²⁺ binding to cyt b₅ H39S leads to a decrease of peroxidase activity. A uranyl binding site was further proposed in the heme-binding domain at Glu37 and Glu43. The impacts of UO₂²⁺ binding to cyt b₅ H39S studied herein provide valuable insights into the toxicity mechanism of UO₂²⁺ towards membrane heme proteins.

• Periodical: Advanced Materials Research (Volumes 455-456)
• Pages: 1204-1209
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.455-456.1204
• Online since: January 2012
• Authors: Dun Wan, Li Fu Liao, Ying Wu Lin
• Keywords: Heme Proteins, Metal-Binding Site, Modeling, Toxicity, Uranium

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