Preparation and Characterization of Fibrous Hydroxyapatite/Chitosan Nanocomposites with High Hydroxyapatite Dosage

Abstract:

In this paper the hydroxyapatite fibers reinforced chitosan nanocomposites with high hydroxyapatite dosage (70~90 wt%) were synthesized by in-situ hybridization. The semi-permeable membrane was used to control the process of hybridization and morphology of hydroxyapatite. The compositional and morphological properties of nanocomposites were investigated by FTIR spectroscopy, X-ray diffraction, and transmission electron microscopy. The results showed that the hydroxyapatite were carbonated nanometer crystalline fibers with high aspect ratio (about 25) and dispersed uniformly in the nanocomposites. The high-resolution image indicated that the growth of nano-hydroxyapatite crystallites in the chitosan matrix preferred in the c-axis. The mechanical properties of these nanocomposites were enhanced dramatically and the compressive strength increases almost to 170MPa when the hydroxyapatite content is 70 wt%. The in vitro tests indicated that the composites have high bioactivity and degradation. These properties illustrated the potential application of this kind of nanocomposites for bone tissue engineering.

• Periodical: Advanced Materials Research (Volumes 457-458)
• Pages: 365-371
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.457-458.365
• Online since: January 2012
• Authors: Cai Yun Zhang, Dai Yin Peng, Chuan Hua Lu, Xian Ping Wang, Qian Feng Fang
• Keywords: Bioactivity, Degradation, Fibers Reinforcement, Fibrous Hydroxyapatite/Chitosan Nanocomposites, In situ Hybridization

References

[1] B.R. Constantz, I.C. Ison, M.T. Fulmer, R.D. Poser, S.T. Smith, M. van Wagoner, J. Ross, S.A. Goldstein, J.B. Jupiter, D.I. Rosenthal, Skeletal repair by in situ formation of the mineral phase of bone, Science. 267(1995) 1796-1799.

DOI: 10.1126/science.7892603

[2] M. Sundfeldt, L.V. Carlsson, C.B. Joansson, P. Thomsen, C. Gretzer, Aseptic loosening, not only a question of wear: a review of different theories, Acta. Orthop. 77 (2006) 177-179.

DOI: 10.1080/17453670610045902

[3] A.J. Tonino, B.C.H. van der Wal, I.C. Heyligers, B. Grimm, Bone remodeling and hydroxyapatite resorption in coated primary hip prostheses, Clin. Orthop. Relat. Res. 467 (2009) 478-484.

DOI: 10.1007/s11999-008-0559-y

[4] J. Jordan, K.I. Jacop, R. Tannenbaum, M.A. Sharaf, I. Jasiuk, Experimental trends in polymer nanocomposites-a review, Mater. Sci. Eng A. 393 (2005) 1-11.

[5] A. Adnan, C.T. Sun and H. Mahfuz, A molecular dynamics simulation study to investigate the effect of filler size on elastic properties of polymer nanocomposites, Compos. Sci. Technol. 67 (2007) 348-356.

DOI: 10.1016/j.compscitech.2006.09.015

[6] M.R. Rogel, H. Qiu, G.A. Ameer, The role of nanocomposites in bone regeneration, J. Mater. Chem. 18(2008) 4233-4241.

DOI: 10.1039/b804692a

[7] P.A. Tran, L. Sarin, R.H. Hurt, T.J. Webster, Opportunities for nanotechnology-enabled bioactive bone implants J. Mater. Chem. 19(2009) 2653-2659.

DOI: 10.1039/b814334j

[8] Z. Ahmad, J. E Mark, Biomimetic materials: recent developments in organic–inorganic hybrids, Mater. Sci. Eng C. 6(1998) 183-196.

[9] M.N. Kumar, R.A. Muzzarelli, C. Muzzarelli, H. Sashiwa, A.J. Domb, Chitosan chemistry and pharmaceutical perspectives, Chem. Rev. 104(2004) 6017-6084.

DOI: 10.1021/cr030441b

[10] J.J. Grodzinski, Biomedical applications of functional polymers, React. Function. Polym. 39(1999) 99-138.

[11] R. Murugan, S. Ramakrishna, In-situ formation of recombinant humanlike collagen-hydroxyapatite nanohybrid through bionic approach, Appl. Phys. Lett. 88 (2006) 193124-193127.

DOI: 10.1063/1.2202138

[12] X. Lu, Y.B. Wang, Y.R. Liu, J.X. Wang, S.X. Qu, B. Feng, J. Weng, Preparation of HA/chitosan composite coatings on alkali treated titanium surfaces through sol-gel techniques, Mater. Lett. 61 (2007) 3970-3973.

DOI: 10.1016/j.matlet.2006.12.089

[13] J. Brugnerotto, J. Lizardi, F.M. Goycoolea, W. Arguelles-Monal, J. Desbrieres, M. Rinaudo, An infrared investigation in relation with chitin and chitosan characterization, Polymer. 42 (2001) 3569-3580.

DOI: 10.1016/s0032-3861(00)00713-8

[14] G. Falini, S. Weiner, L. Addadi, Chitin-silk fibroin interactions: Relevance to calcium carbonate formation in invertebrates, Calcif. Tissue. Int. 72 (2003) 548-554.

DOI: 10.1007/s00223-002-1055-0

[15] G. Penel, G. Leroy, C. Rey, B. Sombret, J.P. Huvenne, E. Bres, Infrared and Raman microspectrometry study of fluor-fluor-hydroxy and hydroxy-apatite powders, J. Mater. Sci. Mater. Med. 8 (1997) 271-276.

DOI: 10.1023/a:1018504126866

[16] I. Rehman, W. Bonfield, Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy, J. Mater. Sci. Mater. Med. 8(1997) 1-4.

[17] I. Yamaguchi, K. Tokuchi, H. Fukuzaki, Y. Koyama, K. Takakuda, H. Monma, J. Tana-ka. Preparation and microstructure analysis of chitosan/hydroxyapatite nanocomposites. J. Biomed. Mater. Res A. 55 (2001) 20-27.

DOI: 10.1002/1097-4636(200104)55:1<20::aid-jbm30>3.0.co;2-f

[18] Powder Diffraction File #9-432, International Centre of Diffraction Data (ICDD), Newton Square, PA, USA.

[19] Q.L. Hu, B.Q. Li, M. Wang, J.C. Shen, Preparation and characterization of biodegradable chitosan/hydroxyapatite nanocomposite rods via in-situ hybridization: a potential material as internal fixation of bone fracture, Biomaterials. 25(2004).

DOI: 10.1016/s0142-9612(03)00582-9

[20] M.G. Ma, Y.J. Zhu, J. Chang, Monetite formed in mixed solvents of water and ethylene glycol and its transformation to hydroxyapatite, J. Phys. Chem B. 110 (2006) 14226-14230.

DOI: 10.1021/jp061738r

[21] G.S. Sailaja, S. Velayudhan, M.C. Sunny, K. Sreenivasan, H.K. Varma, P. Ramesh, Hydroxyapatite filled chitosan-polyacrylic acid polyelectrolyte complexes, J. Mater. Sci. 38 (2003) 3653-3662.

DOI: 10.1023/a:1025689701309

[22] X.H. Wang, J.B. Ma, Y.N. Wang, B.L. He, Structural characterization of phosphorylated chitosan and their applications as effective additives of calcium phosphate cements, Biomaterials. 22 (2001) 2247-2255.

DOI: 10.1016/s0142-9612(00)00413-0

[23] J. Park, R. Lakes, Biomaterials-An Introduction, 2nd ed, Plenum Press, New York, (1992).

[24] K. Hirotak, Biomed. Mater. Res. Far. East (I), Kyoto, Kobunshi Kankokai Inc, 1993, p.35.