Separation and Detection of Monodisperse Polystyrene Nanospheres by Capillary Electrophoresis

Article Preview

Abstract:

Monodisperse polystyrene nanospheres with different diameters and functional groups were synthesized by soap-free emulsion polymerization. Capillary electrophoresis (CE) was applied to the separation and detection of the nanospheres. Baseline separation was achieved for carboxylic and sulfonic functionalized colloids due to their difference in diameter and surface charge. The electrophoretic mobility of the sulfonic functionalized colloids decreased with increasing of particle size, and three sulfonic functionalized nanosphere samples with diameters of 190, 250 and 330 nm were separated successfully by CE.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

51-55

Citation:

Online since:

February 2012

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2012 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] Y.H. Rezenom, A.D. Wellman, L. Tilstra, C.D. Medley, S.D. Gilman, Separation and detection of individual submicron particles by capillary electrophoresis with laser-light-scattering detection, Analyst. 132 (2007) 1215–1222.

DOI: 10.1039/b709509k

Google Scholar

[2] W.J. Chen, K. Mou, B. Xu, X. M. Ling, J. R. Cui, P. Xu, Capillary electrophoresis for screening of 20S proteasome inhibitors, Analytical Biochemistry 394 (2009) 62–67.

DOI: 10.1016/j.ab.2009.07.020

Google Scholar

[3] E. Hurtado-Fernandez, M. Gomez-Romero, A. Carrasco-Pancorbo, A. Fernandez-Gutierrez, Application and potential of capillary electroseparation methods to determine antioxidant phenolic compounds from plant food material, Journal of Pharmaceutical and Biomedical Analysis. 53 (2010).

DOI: 10.1016/j.jpba.2010.07.028

Google Scholar

[4] M. Horka, F. Ruzicka, A. Kubesova, V. Hola, K. Slais, Capillary electrophoresis of conidia from cultivated microscopic filamentous fungi, Anal. Chem. 81 (2009) 3997–4004.

DOI: 10.1021/ac900374v

Google Scholar

[5] D. Andreyev, E. A. Arriaga, Simultaneous laser-induced fluorescence and scattering detection of individual particles separated by capillary electrophoresis, Anal. Chem. 79 (2007) 5474–5478.

DOI: 10.1021/ac070770u

Google Scholar

[6] F.K. Liu, Extremely highly efficient on-line concentration and separation of gold nanoparticles using the reversed electrode polarity stacking mode and surfactant-modified capillary electrophoresis, Analytica Chimica Acta. 694 (2011) 167–173.

DOI: 10.1016/j.aca.2011.03.056

Google Scholar

[7] J.S. Baker, L.A. Colon, Influence of buffer composition on the capillary electrophoretic separation of carbon nanoparticles, Journal of Chromatography A. 1216 (2009) 9048–9054.

DOI: 10.1016/j.chroma.2009.08.063

Google Scholar

[8] D.T.R. Stewart, M. D Celiz, G. Vicente, L.A. Colon, D.S. Aga, Potential use of capillary zone electrophoresis in size characterization of quantum dots for environmental studies, Trends in Analytical Chemistry. 30 (2011) 113–122.

DOI: 10.1016/j.trac.2010.10.005

Google Scholar

[9] N.G. Vanifatova, B.Y. Spivakov, J. Mattusch, R. Wennrich, Size separation of silica nanospheres by means of capillary zone electrophoresis, Talanta. 59 (2003) 345–353.

DOI: 10.1016/s0039-9140(02)00523-4

Google Scholar

[10] M.B. Muller, J.P. Quirino, P.N. Nesterenko, P.R. Haddad, S. Gambhir, D. Li, G.G. Wallace, Capillary zone electrophoresis of graphene oxide and chemically converted graphene, Journal of Chromatography A. 1217 (2010) 7593–7597.

DOI: 10.1016/j.chroma.2010.09.069

Google Scholar

[11] S.P. Radko, M. Stastna, A. Chrambach, Capillary zone electrophoresis of sub-μm-sized particles in electrolyte solutions of various ionic strengths: size-dependent electrophoretic migration and separation efficiency, Electrophoresis. 21 (2000).

DOI: 10.1002/1522-2683(200011)21:17<3583::aid-elps3583>3.0.co;2-q

Google Scholar

[12] H.L. Cong, W.X. Cao, Colloidal crystallization Induced by capillary force, Langmuir. 19 (2003) 8177–8181.

DOI: 10.1021/la0344480

Google Scholar

[13] L.L. Yang, H.L. Cong, W.X. Cao, Preparation of mono-dispersed colloids of p(st-mma-spmap) by soap-free emulsion copolymerization, ACTA POLYMERICA SINICA. 4 (2005) 223–226.

Google Scholar