Effect of the Lactide/Glycolide Ratio and Molecular Weight of PLGA on the Bovine BMPs Microspheres Systems

Abstract:

The present investigation was aimed at optimization of BMPs loaded PLGA microspheres formulations resulting in improved encapsulation efficiency and sustained release of BMPs by varying the molecular weight and copolymer composition of PLGA. Double-emulsion solvent evaporation method was used to prepare the microspheres. The effect of polymer molecular weight and copolymer composition on particle properties and release behavior in vitro was reported. The particle size and encapsulation efficiency increased with increase in molecular weight and lactide content of PLGA. While BMPs release in vitro decreased with increase in molecular weight and lactide content of PLGA. SEM pictures revealed that almost all microspheres were spherical but internal morphology was different. The morphology of PLGA microspheres with exorbitant molecular weight(100kD) was anomalistic whereas the morphology of PLGA microspheres with higher glycolide content(50) have porous structures.

• Periodical: Advanced Materials Research (Volumes 466-467)
• Pages: 405-410
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.466-467.405
• Online since: February 2012
• Authors: Z.H. Li, Ji Min Wu, Y.L. Zhao, J. Guan, S.J. Huang, R.X. Li, X.Z. Zhang
• Keywords: Bone Morphogenetic Proteins, Composition, Controlled Release, Molecular Weight, Poly(lactide-co-glycolide)

References

[1] EH Groeneveld, EH Burger: European Journal of Endocrinology Vol. 142(2000), p.9.

[2] GB Wei, QM Jin, WV Giannobile, PX Ma: Biomaterials Vol. 28 (2007), p. (2087).

[3] Gautschi OP, Frey SP, Zellweger R. ANZ: J Surg Vol. 77(2007), p.626.

[4] Winn SR, Uludag H, Hollinger JO: Clin Orthop Relat Res Vol. 367(1999l), p. S95.

[5] Jeppsson C, Boström M, Aspenberg P: Acta Orthop Scand Vol. 70(1999), p.77.

[6] Naoto Saito, Kunio Takaoka: Biomaterials Vol. 24(2003) , pp.2287-2293.

[7] Yamamoto M, Takahashi Y, Tabata Y; Biomaterials Vol. 24(2003) , p.4375.

[8] Kim BS, Mooney DJ: Trends Biotechnol Vol. 16(1998) , p.224.

[9] Whang K, Healy KE, Elenz DR, et al: Tissue Eng Vol. 5(1999) , p.35.

[10] Kellomaki M, Niiranen H, Puumanen K, et al: Biomaterials Vol. 21(2000) , p.2495.

[11] Wallkamm B, Schmid J, Hammerle CH, et al: Clin Oral Implants Res Vol. 14(2003), p.734.

[12] Cornell CN, Lane JM: Clin Orthop. Vol. 355(1998), p.267.

[13] Duggirala SS, Mehta RC, DeLuca PP: Pharm Dev Technol Vol. 1(1996), p.11.

[14] Schrier JA, Fink BF, Rodgers JB, et al: AAPS PharmSciTech Vol. 2(2001), p.73.

[15] Lee SH, Shin H: Adv Drug Deliv Rev Vol. 59(2007), p.339.

[16] Hsu HP, Zanella JM, Peckham SM, et al: J Orthop Res Vol. 24(2006), p.1660.

[17] Jansen JA, Vehof JWM, Ruhe PQ, et al: J Control Release Vol. 101(2005), p.127.

[18] Keskin DS, Tezcaner A, Korkusuz P, et al: Biomaterials Vol. 26(2005), p.4023.

[19] Duggirala SS, Mehta RC, DeLuca PP: Pharm Dev Technol Vol. 1(1996), p.11.

[20] Schrier JA, DeLuca PP: Pharm Dev Technol Vol. 4(1999), p.611.

[21] Schrier JA, DeLuca PP: AAPS Pharm-SciTech Vol. 2(2001), p.66.

[22] Blanco-Prieto MJ, Campanero MA, Besseghir K, et al: J Control Release, Vol. 96 (2004) , p.437.

[23] RA Jain: Biomaterials, Vol. 21(2000), p.2475.

[24] T Mogi, N Ohtake, M Yoshida, et al: Colloids and Surfaces B: Biointerfaces, Vol. 17(2000), p.153.

[25] J Panyam, MM Dali, SK Sahoo, et al: J. Control. Release, Vol. 92(2003), p.173.

[26] J Braunecker, M Baba, GE Milroy, et al: International Journal of Pharmaceutics, Vol. 282(2004), p.19.

[27] R Jalil, JR Nixon: J. Microencapsul. Vol. 7(1990), p.357.

[28] TG Park: Biomaterials, Vol. 16(1995), p.1123.

[29] WK Lee, JY Park, EH Yang, et al: J. Control. Release, Vol. 84(2002), p.115.