Modified Gelatin-Based Cell Assembling Process Using Glycerin

Hai Xia Liu; Sheng Jie Li; Feng Lin; Yong Nian Yan

doi:10.4028/www.scientific.net/AMR.476-478.443

Paper Titles

Research of Ultrasound and Electromagnetic Field on the Microstructure of Cast-Rolling Magnesium Strip
p.426

Comparison on the Compositions, Microstructure and Hardness of Super Large Section Prehardened Steels for Plastic Mould Block
p.430

MD Simulation of Chip Formation in Nanometric Cutting of Metallic Glass
p.434

Design and Simulation of Drum Solid-State Fermentation Equipment Based on Solidworks
p.438

Modified Gelatin-Based Cell Assembling Process Using Glycerin
p.443

Numerical Simulation on Effect of Nozzle-Hole Layout on Spray Characteristics and Combustion in a DI Diesel Engine
p.448

Quantitative Analysis of Inclusions in Aluminum
p.453

Fabrication and Characterization of Hydroxyapatite Nanocomposites Based on Bacterial Cellulose with Surface Modification
p.457

The Study on the Change of Sheer Strength Characteristic Parameter and Soil Nail Firmness of Effect in the Condition of Freezing-Thaw Cycling
p.461

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 476-478Modified Gelatin-Based Cell Assembling Process...

Modified Gelatin-Based Cell Assembling Process Using Glycerin

Abstract:

Cell assembly technology adopting the gelatin-based composite materials has found broad application in the field of disease mechanism research, drug development and organ reconstruction etc. But there are still several troublesome problems, such as the necessaries of high forming concentration of gelatin-based materials and the cell damage produced during extrusion. In view of existing situation, a modified gelatin-based cell assembling process using glycerin was brought forward. The results showed that adding 10% (v/v) glycerin to the existing gelatin-based composite materials, the cells inactivation effect under 4 °C or lower temperature environment can be reduced obviously, meanwhile, the glycerin has a compensatory effect of gelatin. It can significantly improve the forming temperature and the cell survival rate, get high cell survival rate even when the scanning speed is on 40 mm/s. In addition, the glycerin is easier to dissolve in culture medium in the tissue analog training process; it is more conducive to the rapid materials degradation, as well as cell proliferation and tissue regeneration. Therefore, modified gelatin-based cell assembly process with glycerin will be more widely used in tissue or organ in vitro assembly process.