Porous Silicon Biosensor for the Detection of Transgenic Cotton of Antifeezing Protein

Yong Zhang; Xiao Yi Lv; Hong Yan Zhang; Tao Jiang; Ji Ma; Zhen Hong Jia

doi:10.4028/www.scientific.net/AMR.485.288

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Porous Silicon Biosensor for the Detection of Transgenic Cotton of Antifeezing Protein
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 485Porous Silicon Biosensor for the Detection of...

Porous Silicon Biosensor for the Detection of Transgenic Cotton of Antifeezing Protein

Abstract:

Genetic engineering has been used extensively for improving cold resistance in plants. In this paper, an optical immunoassay was developed by the optic reflectance spectroscopy of the porous silicon biosensor to monitor the success of transgenic frost-resistant cotton. Transgenic cotton extract samples and anti- antifreeze protein (AFP) antibodies were employed as the target and probe, respectively. Porous silicon is functionalized by using oxidation and silanization, then anti- AFP antibodies were immobilized to the porous silicon pore used a crosslink method. The changes of reflectance spectrum before and after antigen-antibody react ion are observed. The results show that transgenic frost-resistant cotton extract samples caused obvious red shift in reflectance spectrum of porous silicon while common cotton was not. Control experiment with negative antibodies shows high specifcity and conventional ELASA and Western experiment were also carried for comparison. Compare to conventional ELASA and Western, porous silicon biosensor is quick and accurate determination for the detection of transgenic cotton of antifeezing protein.

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Periodical:

Advanced Materials Research (Volume 485)

Pages:

288-292

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.485.288

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Online since:

February 2012

Authors:

Yong Zhang, Xiao Yi Lv, Hong Yan Zhang, Tao Jiang, Ji Ma, Zhen Hong Jia

Keywords:

Antifreeze Protein, Biosensor, Label-Free Detection, Porous Silicon

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