Immobilization of Porcine pancreatic Lipase on Vesicle-Like Silica Material with Large Pore

Cui Cui Wu; Guo Wei Zhou; De Lan Xu; Tian Duo Li

doi:10.4028/www.scientific.net/AMR.485.314

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 485Immobilization of Porcine pancreatic Lipase on...

Immobilization of Porcine pancreatic Lipase on Vesicle-Like Silica Material with Large Pore

Abstract:

Vesicle-like silica (VS) was prepared by utilizing cationic surfactant cetyltrimethylammonium bromide (CTAB) and anionic surfactant sodium dodecyl sulfate (SDS) as a dual-template. The mesostructures of VS were characterized by N₂ adsorption-desorption isotherms, field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM). Results showed that mean mesopore size of unilayer and multilayer VS was about 14 nm and shell thickness was nearly 6 nm. The VS was chosen as support for immobilization of Porcine pancreatic lipase (PPL) by physical adsorption. The immobilized PPL possessed excellent thermal stability and reusability in the hydrolysis reaction of triacetin in phosphate buffer solution.

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Periodical:

Advanced Materials Research (Volume 485)

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314-317

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.485.314

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Online since:

February 2012

Authors:

Cui Cui Wu, Guo Wei Zhou, De Lan Xu, Tian Duo Li

Keywords:

Activity, Immobilisation, Porcine pancreatic Lipase, Vesicle-Like Silica

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