Rapid Determination of Fumonisin B1 in Food Samples by a Clean-Up Tandem Immunoassay Column

Yan Wang; Guang He Wu; Wei Sheng; Yan Zhang; Meng Yuan; Shuo Wang

doi:10.4028/www.scientific.net/AMR.488-489.1568

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 488-489Rapid Determination of Fumonisin B1 in Food...

Rapid Determination of Fumonisin B1 in Food Samples by a Clean-Up Tandem Immunoassay Column

Abstract:

An immunochemistry-based assay for non-instrumental simultaneous detection of fumonisins in food was developed. The method was based upon the direct competitive immuno-reaction and the horse radish peroxidase enzymatic reaction. The assay was developed to show a visual detection result, according to a yes/no response to the LOD of fumonisins. The limit of detection (LOD) was 40 μg L^-1. The assay could be accomplished within 15 min in all and 4 min for chromogenic substrate application. The fumonisin contaminations in different kinds of food were analyzed by the proposed method and the results were confirmed by ELISA. Avoiding time-consuming reaction steps and complicated pre-treatment procedures, this assay was demonstrated as a promising tool for on-site sample detections.

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Periodical:

Advanced Materials Research (Volumes 488-489)

Pages:

1568-1573

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.488-489.1568

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Online since:

March 2012

Authors:

Yan Wang, Guang He Wu, Wei Sheng, Yan Zhang, Meng Yuan, Shuo Wang

Keywords:

Fumonisins, Non-Instrumental, Rapid Detection, Visual Detection

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