Gelatin/Fibroin Hydrogel for Controlled Release of Human Dentin Matrix Extract

W. Anuchiracheewa; P. Pavasant; Sorada Kanokpanont

doi:10.4028/www.scientific.net/AMR.506.505

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 506Gelatin/Fibroin Hydrogel for Controlled Release of...

Gelatin/Fibroin Hydrogel for Controlled Release of Human Dentin Matrix Extract

Abstract:

Dentin matrix extract (DME) was reported to induce differentiation of bone cell line MC3T3-E1, since it contained a number of growth factors. Human DME was prepared from human teeth milled under liquid nitrogen. The resulted powder was demineralized in 0.5M EDTA and the crude proteins were separated using dialysis. DME contained proteins with molecular weight in the range of 55-72 kDa and had Zeta-potential value at-10.53 ± 0.12 mV, and-13.53 ± 0.12 mV, in PBS (pH 7.5), and in deionized water (pH 5.5), respectively. Three types of hydrogel carriers were fabricated from gelatin and Thai silk fibroin (G:SF) at the weight ratio of 100:0, 70:30, and 50:50. Glutaraldehyde was used as crosslinker. The carriers had water absorption 90.43 ± 0.29 %, 87.73 ± 0.29 %, 86.60 ± 0.76 % by weight respectively. In vitro release experiment of DME from these carriers showed controlled-release mechanism. The G:SF 100:0 released the highest DME content within 48 h, while the G:SF 50:50 hardly released DME within 2 days. These results shows the G:SF system can be used for controlled-release for DME.