The effect of morphology and distribution of sulfides on tensile, impact and bending fatigue properties of non-quenched and tempered steel 49MnVS3 has been investigated in this paper. Microscopic structure and morphology of sulfides are observed, and impact fracture and fatigue fracture have been analyzed by SEM. The results show that the morphology of sulfides is mostly strip and distributes in ferrite, which affects mechanical properties and fatigue life. The length direction of sulfide strip is parallel to the rolling direction of steel. When the length of sulfide is short relatively and is approximate to the shape of particles. The impact properties and bending fatigue performance of 49MnVS3 are higher. Under those conditions, there are more ductile characteristics in their impact fracture and the fatigue fracture. The reasons for the effect of sulfide morphology on the mechanical and fatigue properties are explained.