Detection to E. Coli O157 by Surface Plasmon Resonance Immunosensor

Hui Yao; Xiao Hui Zhang; Hong Zong Yin

doi:10.4028/www.scientific.net/AMR.518-523.305

Paper Titles

Adsorption of Cr(VI) onto the Cross-Linked Cationic Starch and the Establishment of the Adsorption Isotherm and Kinetic Equation
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Comparison of Different Types of Redox Mediators on the Chemical Reduction of Azo Dye by Sulfide
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Degradation of P-Nitrophenol Affected by Synergy of Indirect and Direct Electrooxidation
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Detection of Methanogens in Anaerobic Granular Sludge by FISH Targeting for Functional Genes
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Detection to E. Coli O157 by Surface Plasmon Resonance Immunosensor
p.305

Determination of Lignocellulase Activity and Gene Expression Using Magnetic Nanoparticle-Based Electrochemical Biosensor
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Different Organic Resources Influence Combined Forms of Humus in Black Soil Aggregation
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Distribution of Organic Matter in Sediment of Lakeside Belt in East Dongting Lake
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Distributions of Carbon Nitrogen and Phosphorus in the Products from SCWG of Cyanobacteria
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 518-523Detection to E. Coli O157 by Surface Plasmon...

Detection to E. Coli O157 by Surface Plasmon Resonance Immunosensor

Abstract:

This work describes an approach for the development of two bacteria biosensors based on surface plasmon resonance (SPR) technique. The first biosensor was based on functionalized gold substrate and the second one on immobilized silver nanoparticles. For the first biosensor, the gold substrate was functionalized with acid–thiol using the self-assembled monolayer technique, while the second one was functionalized with silver nanoparticles immobilized on modified gold substrate. A polyclonal anti-Escherichia coli antibody was immobilized for specific (E. coli) bacteria detection. Detection limit with a good reproducibility of 104 and 103 cfu•mL−1 of E. coli bacteria has been obtained for the first biosensor and for the second one respectively.

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Advanced Materials Research (Volumes 518-523)

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305-308

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.518-523.305

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Online since:

May 2012

Authors:

Hui Yao, Xiao Hui Zhang, Hong Zong Yin

Keywords:

Biofunctionalization, Immunosensor, Silver Nanoparticle, Surface Plasmon Resonance (SPR)

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