Molecular Cloning and Characterization Analysis of Phosphofructokinase Gene from Dunaliella parva

Chang Hua Shang; Zhong Ming Wang; Shun Ni Zhu; Lei Qin; Zhen Hong Yuan

doi:10.4028/www.scientific.net/AMR.518-523.73

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 518-523Molecular Cloning and Characterization Analysis of...

Molecular Cloning and Characterization Analysis of Phosphofructokinase Gene from Dunaliella parva

Abstract:

Phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6- bisphosphate, a key regulatory step in the glycolytic pathway. The former studies indicated the PFK could enhance glycolysis. The full-length cDNA encoding PFK was obtained from oleaginous microalgae Dunaliella parva, which include 1572 bp open reading frame (ORF), 254 bp 5′-untranslated sequence and 323 bp 3′-untranslated sequence. Dunaliella parva PFK showed the highest sequence similarity with the PFK from Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva PFK also showed wide similarity with other species.

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Advanced Materials Research (Volumes 518-523)

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73-76

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.518-523.73

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Online since:

May 2012

Authors:

Chang Hua Shang, Zhong Ming Wang, Shun Ni Zhu, Lei Qin, Zhen Hong Yuan

Keywords:

Dunaliella Parva, Molecular Cloning, Phosphofructokinase, Sequence Analysis

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