Biocompatibility of Polyhydroxyalkanoates Synthesized by Bacillus cereus BMH

Ming Hong Bian; Wen Feng Hu; Xu Pang; Yong Zuo

doi:10.4028/www.scientific.net/AMR.531.423

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 531Biocompatibility of Polyhydroxyalkanoates...

Biocompatibility of Polyhydroxyalkanoates Synthesized by Bacillus cereus BMH

Abstract:

Polyhydroxyalkanoates (PHAs) are biological polyesters synthesized by many bacteria. The polyesters became the focus of research because of their biodegradability, biocompatibility and the similar physical and chemical properties to those of petroleum derived plastics. A gram-positive strain was isolated from domesticated activated sludge and identified as Bacillus cereus BMH. B. cereus BMH can synthesize PHAs using a wide range of carbon sources. After 36h cultivation in shaken flasks, cell dry-weight was 5.31g/l, PHAs content was 61.2% of cell dry weight which is higher than some other B. cereus reported. The PHAs films showed irregular porous structure under scanning electron microscopy. Biocompatibility of the polyester was evaluated in detail in this article. The polymer was proved of good biocompatibility through Scanning Electron Microscopy (SEM), Fluorescence microscopy observation and CCK-8 assay. It was indicated that the biological polymer had desirable material properties as tissue engineering biomaterials

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Periodical:

Advanced Materials Research (Volume 531)

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423-427

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https://doi.org/10.4028/www.scientific.net/AMR.531.423

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Online since:

June 2012

Authors:

Ming Hong Bian, Wen Feng Hu, Xu Pang, Yong Zuo

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Bacillus cereus, Biocompatibility, Biodegra-Dable Material, Polyhydroxyalkanoates

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References

[1] Madison, L.L., and Huisman, G.W. Metabolic engineering of poly- (3-hydroxyalkanoates): from DNA to plastic. Microbial. Mol. Biol. Vol. 63 (1999), p.21–53.

DOI: 10.1128/mmbr.63.1.21-53.1999

Google Scholar

[2] Kim, Y.B., and Lenz, R.W. Polyesters from microorganisms. Adv. Biochem. Eng. Biotechnol. vol. 71(2001), p.51–79.

Google Scholar

[3] Reddy, C.S., Ghai, R., and Rashmi, Kalia, V.C. Polyhydroxyalkanoates: an overview. Bioresour. Technol. vol. 87(2003), p.137–146.

DOI: 10.1016/s0960-8524(02)00212-2

Google Scholar

[4] Chien, C. C., C. C. Chen, M. H. Choi, S. S. Kung, and Y. H. Wei. Production of poly-beta-hydroxybutyrate (PHB) by Vibrio spp. isolated from marine environment. J. Biotechnol. vol. 132 (2007), pp.259-263.

DOI: 10.1016/j.jbiotec.2007.03.002

Google Scholar

[5] Chua H., Yu P.H.F., Xing S. and Ho L.Y. Potential of biodegradable plastics as environmentally-friendly substitutes for conventional plastics in Hong Kong. Presented at the 17th Sympossium on Biotechnology for Fuels and Chemicals, Colorado, U.S.A. (1995).

Google Scholar

[6] Reusch R N. Poly-β-hydroxybutyrate calium polyphos- phate complexes in eukaryotic membranes. Process in Polymer Science. vol. 191(1981), pp.377-381.

Google Scholar

[7] Luengo, J.M., Garcia, B., Sandoval, A., Naharro, G., Olivera, E.R. Bioplastics from microorganisms. Curr. Opin. Microbiol. vol. 6(2003), p.251–260.

DOI: 10.1016/s1369-5274(03)00040-7

Google Scholar

[8] Hahn, S.K., Chang, Y.K., Kim, B.S., Chang, H.N. Optimization of microbial poly (3-hydroxybutyrate) recovery using dispersions of sodium hypochlorite solution and chloroform. Biotechnol. Bioeng. vol. 44(1944), p.256–261.

DOI: 10.1002/bit.260440215

Google Scholar

[9] Hahn, S.K., Chang Y.K., Kim, B.S., Lee, K.M., Chang, H.N. The recovery of poly(3-hydroxybutyrate) by using dispersion of sodium hypochlorite solution and chloroform. Biotechnol. Tech. vol. 7(1993), p.209–212.

DOI: 10.1007/bf02566149

Google Scholar

[10] Hahn, S.K., Chang, Y.K., Lee, S.Y. Recovery and characterization of poly(3-hydroxybutyric acid) synthesized in Alcaligens eutrophus and recombinant, Escherichia coll. Appl. Environ. Microbiol. vol. 61(1995), p.34–39.

DOI: 10.1128/aem.61.1.34-39.1995

Google Scholar

[11] Chen GQ. A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry. Chem Soc Revvol, vol. 38(2009), pp.2434-2446.

DOI: 10.1039/b812677c

Google Scholar

[12] Dong Y, Li P, Chen C, Wang ZH, Ma P, Chen GQ. The improvement of fibroblast growth on hydrophobic biopolyesters by coating with polyhydroxyalkanoate granule binding protein PhaP fused with cell adhesion motif RGD. Biomaterials, vol 31(2010).

DOI: 10.1016/j.biomaterials.2010.08.001

Google Scholar

[13] Valappil, S.P., Boccaccini, A.R., Bucke, C., Roy, I., a. Polyhydroxyalkanoates in Gram-positive bacteria: insights from the general Bacillus and Streptomyces. Antonie van Leeuwenhoek, vol. 91(2007), pp.1-17.

DOI: 10.1007/s10482-006-9095-5

Google Scholar

[14] Halami, P. M. Production of polyhydroxyalkanoate from starch by the native isolate Bacillus cereus CFR06. World J Microbiol. Biotechnol., vol. 24(2008), pp.805-812.

DOI: 10.1007/s11274-007-9543-z

Google Scholar