Ionic Liquid-Regenerated Cellulose Beads as Solid Support Matrices for Papain Immobilization

Song Kun Yao; Qiu Jin Li; Wei Zhang; Ji Xian Gong; Jian Fei Zhang

doi:10.4028/www.scientific.net/AMR.535-537.2349

Paper Titles

Optimization of the Extraction of Coconut Protein and the Evaluation of Antioxidant and ACE Inhibitory Activities of its Enzymatic Hydrolysates
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Chemical Analysis and Antioxidant Activities In Vitro of Polysaccharide Extracted from Corn Silk
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Preparation and Electrospinning of Acidified-Oxidized Potato Starch
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Formation of Silkworm-Like Vaterite via Orientated Attachment Process
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Ionic Liquid-Regenerated Cellulose Beads as Solid Support Matrices for Papain Immobilization
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Producing Cellulase on Phanerochaete chrysosporium by Fermentation and Breeding by Induced Mutation of Cellulose-Degrading Phanerochaete chrysosporium
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Study on Biocompatibility of Post-Irradiated Silk Fibroin In Vitro
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Effect of Gamma Irradiation on the Biocompatibility and Biodegradation of Silk Fibroin In Vivo
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 535-537Ionic Liquid-Regenerated Cellulose Beads as Solid...

Ionic Liquid-Regenerated Cellulose Beads as Solid Support Matrices for Papain Immobilization

Abstract:

A beads based on cellulose and the room temperature ionic liquid 1-butyl-3-methyl imidazolium chloride ([Bmim]Cl) was prepared. Regenerated cellulose beads were modified with silane, and characterized by scanning electron microscopy. Papain was immobilized on the beads used two different methods including glutaraldehyde and covalent cross-linking method. The immobilized enzyme activity of bead was determinated by BAEE (N-benzoyl- DL-arginine ethyl ester hydrochloride) determination. According to the enzyme activity and immobilization rate compared with covalent cross-linking method, glutaraldehyde cross-linking method is more suitable for amino-modified.

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Advanced Materials Research (Volumes 535-537)

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2349-2352

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.535-537.2349

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Online since:

June 2012

Authors:

Song Kun Yao, Qiu Jin Li, Wei Zhang, Ji Xian Gong, Jian Fei Zhang

Keywords:

Beads, Cellulose, Enzyme Immobilization, Ionic Liquid (IL), Papain

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