Optimization of the Extraction of Coconut Protein and the Evaluation of Antioxidant and ACE Inhibitory Activities of its Enzymatic Hydrolysates

Zhe Liu; Xuan Ri Shen; Yang Yu; Yao Wang; Peng Li

doi:10.4028/www.scientific.net/AMR.535-537.2330

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 535-537Optimization of the Extraction of Coconut Protein...

Optimization of the Extraction of Coconut Protein and the Evaluation of Antioxidant and ACE Inhibitory Activities of its Enzymatic Hydrolysates

Abstract:

The extraction of proteins from coconut meat was performed utilizing alkaline extraction. On the basis of single factor experiment, an orthogonal experiment was designed to optimize the extraction conditions (including extraction time, temperature, NaOH concentration and liquid to solid ratio). The highest extraction rate (7.3%) was obtained at 120 min, 45 °C, 0.1 M and liquid-solid ratio 25:1. Coconut protein was consequently hydrolyzed with four commercial enzymes (alcalase, trypsin, pepsin, papain). Furthermore, activities of hydrolysates on DPPH radical scavenging and ACE inhibition were assayed respectively. The results indicated that pepsin hydrolysate exhibited the highest DPPH radical scavenging activity (100%, 1 mg protein/mL), and trypsin hydrolysate possessed the most potent ACE inhibitory activity (40.2%, 1 mg protein/mL).

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Advanced Materials Research (Volumes 535-537)

Pages:

2330-2334

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.535-537.2330

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Online since:

June 2012

Authors:

Zhe Liu, Xuan Ri Shen, Yang Yu, Yao Wang, Peng Li

Keywords:

ACE Inhibitory Activity, Alkaline Extraction, Coconut Protein, DPPH Radical Scavenging Activity, Extraction Ratio

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